We have developed a novel nerve holder, imaging protocol and post-processing routine which enhance OCT imaging by accessing peripheral nerve from 3 sides. Two mirror prisms provide imaging access from sides of a peripheral nerve in addition to conventional volume acquisition from the top. Prism glass compensates for changed focusing and optical pathlength during nerve imaging from its sides Acquired prism sub-volumes and top-bottom imaged nerve sub-volume are merged in two-stage semi-automated postprocessing routine. We demonstrate successful application of this approach on tissue-mimicking phantoms, as well as in vivo rat sciatic nerve imaging.
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