This study reports the effects of substrate temperature and laser ablation wavelength on the structural and bioactivity properties of hydroxyapatite (HAP) coatings. The coatings were obtained using a pulsed laser deposition technique on Ti6Al4V and Si(100) substrates. Different substrate temperatures were used ranging from 25°C to 800°C. Three wavelengths of Nd:YAG pulsed laser (1064 nm, 532 nm, and 355 nm) were employed to study the ablation mechanisms and their effects on film morphology. Surface morphology was investigated by SEM with EDX Analysis and AFM. All coatings were confirmed to be grown in a granular system and it was observed that 355 nm and 532 nm produce smoother coatings. The XRD measurements showed the transition from amorphous to crystalline HAP beyond 500°C. The adhesion strength of the coatings to the substrates was analyzed by pull-out tests. Although as substrate temperature increased, adhesion also got better, further increase of temperature to 800 °C resulted in a significant decrease in bonding. Finally, the bioactivity of the coatings was assessed on multiple levels such as protein adsorption, dissolution in simulated body fluid, and cell proliferation.
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