To investigate whether endogenous biliverdins can serve as a fluorescence metabolic marker in cancer diagnosis, we
measured their multiphoton fluorescence spectra and lifetimes with femtosecond Cr:forsterite laser. Excited at 1230nm,
the two-photon fluorescence of biliverdins peaks around 670nm. The corresponding lifetime (<100ps) was much shorter
than those of porphyrins (~10ns), which is another commonly present metabolites in living cells. Further mixing
biliverdins with proteins like fetal bovine serum (FBS), biliverdins reductase A (BVRA), or heme oxygenase-1 (HO-1),
the yields of red autofluorescences didn't change a lot, but the corresponding lifetimes with HO-1 and BSA were
lengthened to 200~300ps. This indicates that biliverdin can have an association with these proteins and change its
lifetime. These spectral and temporal characteristics of fluorescence make biliverdin a potential marker fluorophore for
hyperspectral diagnosis on the heme catabolism in human cells or tissues.
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