Dr. Christine S. Mulvey Profile

Dr. Christine S. Mulvey

Instructor/Lab Admin at Duke Univ

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Publications (5)

SPIE Journal Paper | 21 June 2016 Open Access

Correlation of breast tissue histology and optical signatures to improve margin assessment techniques

Stephanie Kennedy, Matthew Caldwell, Torre Bydlon, Christine Mulvey, Jenna Mueller, Lee Wilke, William Barry, Nimmi Ramanujam, Joseph Geradts

JBO, Vol. 21, Issue 06, 066014, (June 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.6.066014

KEYWORDS: Collagen, Scattering, Tissues, Tissue optics, Breast, Brain-machine interfaces, Biomedical optics, Model-based design, Mie scattering, Pathology

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SPIE Journal Paper | 1 November 2011 Open Access

Wavelength-dependent backscattering measurements for quantitative monitoring of apoptosis, Part 2: early spectral changes during apoptosis are linked to apoptotic volume decrease

Christine Mulvey, Wei-Han Liu, Irving Bigio, Kexiong Zhang, David Waxman

JBO, Vol. 16, Issue 11, 117002, (November 2011) https://doi.org/10.1117/12.10.1117/1.3644911

KEYWORDS: Cell death, Scattering, Backscatter, Signal attenuation, Mie scattering, Solids, Flow cytometry, Scatter measurement, Water, Spectroscopy

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SPIE Journal Paper | 1 November 2011 Open Access

Wavelength-dependent backscattering measurements for quantitative monitoring of apoptosis, Part 1: early and late spectral changes are indicative of the presence of apoptosis in cell cultures

Christine Mulvey, Wei-Han Liu, Irving Bigio, Kexiong Zhang, David Waxman

JBO, Vol. 16, Issue 11, 117001, (November 2011) https://doi.org/10.1117/12.10.1117/1.3644389

KEYWORDS: Cell death, Scattering, Backscatter, Cancer, Scatter measurement, Biomedical optics, Ocean optics, Spectroscopy, Signal processing, Oncology

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SPIE Journal Paper | 1 November 2009 Open Access

Wavelength-dependent backscattering measurements for quantitative real-time monitoring of apoptosis in living cells

Christine Mulvey, Carly Sherwood, Irving Bigio

JBO, Vol. 14, Issue 06, 064013, (November 2009) https://doi.org/10.1117/12.10.1117/1.3259363

KEYWORDS: Cell death, Scattering, Particles, Backscatter, Mie scattering, Light scattering, Scatter measurement, Cancer, Spectroscopy, Tissues

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Apoptosis-programmed cell death-is a cellular process exhibiting distinct biochemical and morphological changes. An understanding of the early morphological changes that a cell undergoes during apoptosis can provide the opportunity to monitor apoptosis in tissue, yielding diagnostic and prognostic information. There is avid interest regarding the involvement of apoptosis in cancer. The initial response of a tumor to successful cancer treatment is often massive apoptosis. Current apoptosis detection methods require cell culture disruption. Our aim is to develop a nondisruptive optical method to monitor apoptosis in living cells and tissues. This would allow for real-time evaluation of apoptotic progression of the same cell culture over time without alteration. Elastic scattering spectroscopy (ESS) is used to monitor changes in light-scattering properties of cells in vitro due to apoptotic morphology changes. We develop a simple instrument capable of wavelength-resolved ESS measurements from cell cultures in the backward direction. Using Mie theory, we also develop an algorithm that extracts the size distribution of scatterers in the sample. The instrument and algorithm are validated with microsphere suspensions. For cell studies, Chinese hamster ovary (CHO) cells are cultured to confluence on plates and are rendered apoptotic with staurosporine. Backscattering measurements are performed on pairs of treated and control samples at a sequence of times up to 6-h post-treatment. Initial results indicate that ESS is capable of discriminating between treated and control samples as early as 10- to 15-min post-treatment, much earlier than is sensed by standard assays for apoptosis. Extracted size distributions from treated and control samples show a decrease in Rayleigh and 150-nm scatterers, relative to control samples, with a corresponding increase in 200-nm particles. Work continues to correlate these size distributions with underlying morphology. (Partial Abstract)

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Proceedings Article | 24 February 2009 Paper

Wavelength-dependent backscattering measurements for quantitative real-time detection of apoptosis in living cells

Christine Mulvey, Carly Sherwood, Irving Bigio

Proceedings Volume 7187, 71870M (2009) https://doi.org/10.1117/12.808207

KEYWORDS: Scattering, Cell death, Particles, Mie scattering, Backscatter, Light scattering, Optical spheres, Refraction, Spectroscopy, In vitro testing

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