Ms. Conghui Shao Profile

Conghui Shao

at Tsinghua Univ

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Author

Publications (5)

SPIE Journal Paper | 30 September 2023 Open Access

Influence of hematoxylin and eosin staining on linear birefringence measurement of fibrous tissue structures in polarization microscopy

Liangyu Deng, Chunyan Chen, Wenxi Yu, Conghui Shao, Zan Shen, Yonggang Wang, Chao He, Hongtao Li, Zhiyan Liu, Honghui He, Hui Ma

JBO, Vol. 28, Issue 10, 102909, (September 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.10.102909

KEYWORDS: Tissues, Biological samples, Birefringence, Bone, Collagen, Polarization, Dyes, Polarimetry, Statistical analysis, Biological imaging

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SignificanceFor microscopic polarization imaging of tissue slices, two types of samples are often prepared: one unstained tissue section for polarization imaging to avoid possible influence from staining dyes quantitatively and one hematoxylin-eosin (H&E) stained adjacent tissue section for histological diagnosis and structural feature identification. However, this sample preparation strategy requires high-quality adjacent tissue sections, and labeling the structural features on unstained tissue sections is impossible. With the fast development of data driven-based polarimetric analysis, which requires a large amount of pixel labeled images, a possible method is to directly use H&E stained slices, which are standard samples archived in clinical hospitals for polarization measurement.AimWe aim to study the influence of hematoxylin and eosin staining on the linear birefringence measurement of fibrous tissue structures.ApproachWe examine the linear birefringence properties of four pieces of adjacent bone tissue slices with abundant collagen fibers that are unstained, H&E stained, hematoxylin (H) stained, and eosin (E) stained. After obtaining the spatial maps of linear retardance values for the four tissue samples, we carry out a comparative study using a frequency distribution histogram and similarity analysis based on the Bhattacharyya coefficient to investigate how H&E staining affects the linear birefringence measurement of bone tissues.ResultsLinear retardance increased after H&E, H, or E staining (41.7%, 40.8%, and 72.5% increase, respectively). However, there is no significant change in the imaging contrast of linear retardance in bone tissues.ConclusionsThe linear retardance values induced by birefringent collagen fibers can be enhanced after H&E, H, or E staining. However, the structural imaging contrasts based on linear retardance did not change significantly or the staining did not generate linear birefringence on the sample area without collagen. Therefore, it can be acceptable to prepare H&E stained slices for clinical applications of polarimetry based on such a mapping relationship.

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SPIE Journal Paper | 7 August 2023 Open Access

Analyzing the influence of oblique incidence on quantitative backscattering tissue polarimetry: a pilot ex vivo study

Zheng Zhang, Conghui Shao, Honghui He, Chao He, Shaoxiong Liu, Hui Ma

JBO, Vol. 28, Issue 10, 102905, (August 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.10.102905

KEYWORDS: Tissues, Polarimetry, Biological samples, Backscatter, Polarization, Liver, Light sources and illumination, Reflection, Monte Carlo methods, Statistical analysis

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Proceedings Article | 15 March 2023 Poster + Paper

Comparative study of the influence of imaging resolution on polarization properties derived from Mueller matrix

Yuxin Zhang, Conghui Shao, Honghui He, Hui Ma

Proceedings Volume 12382, 123820A (2023) https://doi.org/10.1117/12.2648648

KEYWORDS: Mueller matrices, Image resolution, Polarization imaging, Polarization, Objectives, Tissues, Polarimetry, Biological samples, Structured optical fibers, Skin

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Proceedings Article | 19 December 2022 Presentation + Paper

Analyzing the influence of imaging resolution on tissue polarimetry basis parameters

Conghui Shao, Honghui He, Hui Ma

Proceedings Volume 12320, 123200D (2022) https://doi.org/10.1117/12.2644094

KEYWORDS: Polarization, Image resolution, Objectives, Tissues, Structured optical fibers, Monte Carlo methods, Scattering, Polarimetry, Microscopes, Skin

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Proceedings Article | 5 March 2021 Presentation + Paper

A quantitative and non-contact technique to detect skin water content based on Mueller matrix polarimetry

Haoyu Zhai, Rongrong Huang, Conghui Shao, Honghui He, Hui Ma

Proceedings Volume 11646, 116460A (2021) https://doi.org/10.1117/12.2577579

KEYWORDS: Skin, Polarimetry, Tissues, Statistical analysis, Mueller matrices, Monte Carlo methods, Collagen, Biological research

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