The detailed 3-D mapping of tissue microcirculation, including blood oxygen saturation
and flow, would provide important biometrics needed to understand the cause and
progression of numerous diseases. To that end we have started developing a two-color
Fourier domain Pump-Probe Optical Coherence Tomography (PPOCT) system designed
specifically to image hemoglobin with the eventual goal of measuring blood oxygen
saturation. This system utilizes a two-color pump-probe scheme chosen to maximize the
potential imaging depth by probing in the near IR where the tissue scattering properties are
most favorable and pumping in the visible where the hemoglobin light absorption is most
efficient. A sample consisting of pure hemoglobin placed between two coverslips has been
used for the initial demonstration and to begin the process of optimizing the system.
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