Current paper presents the results of the chlorine e6 (Ce6) study on 2D and 3D models of FaDu cells culture. The 2D model or monolayer was used for investigation of Ce6 distribution within individual cells and their organelles. The 3D model or multicellular tumor spheroids were used for estimation of cells’ metabolic processes by the investigation of the Ce6 fluorescence distribution within spheroid's layers and Ce6 fluorescence lifetime. It was shown that 3D cell cultures and Сe6 allows estimating the cells’ metabolic processes better than in 2D monolayer cell cultures. Also, this model allows estimating the photodynamic effect depending on the proximity to the surface of different areas inside the heterogeneous 3D structure.
Today the most important problem of a transplantation is a rejection of healing skin tissue. The reason of a skin rejection is a high level of inflammation reaction and a slow rate of neoangeoginesis. A lot of methods exist for imaging of tissue healing extent, unfortunately, all of them have some drawbacks. Laser induced fluorescence is a non-invasive method which provides ambulatory and fast diagnosis. The concept was created and optimal parameters of spectral device were selected based on the experiment results. The non-invasive spectral device will allow determining a state of a healing skin and rate of skin tissue engraftment or rejection by its spectroscopic properties analysis using aluminum phthalocyanine nanoparticles (nAlPc). These nanoparticles are spectroscopically sensitive to inflammation reactions and begin to fluoresce while interacting with immune cells in inflamed tissue. The operation principle of developed device based on analysis of diffuse reflected light from a skin area. The device consists of the six red laser diodes. The red range laser irradiation allows dedicating autofluorescence of biological tissue components such as lipopigments, porphyrins. The fluorescence intensity of exogenous fluorophores helps identifying the degree of transplant engraftment because it is correlate with the inflammatory reactions intensity in a skin. End users will be burn centers, medicine facilities for monitoring of a postoperative sutures engraftment. It can also be used at home to assess the healing of small wounds.
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