Dr. Dominique Dumas Profile

Dr. Dominique Dumas

at Nancy-Univ

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Publications (7)

Proceedings Article | 13 July 2009 Paper

Prevention of bladder tumor implantion after fluorescence-guided TUR with photodynamic therapy

Saoussen Berrahmoune, Lina Bezdetnaya, Peter de Witte, Agnès Leroux, Dominique Dumas, François Guillemin, Marie Ange D'Hallewin

Proceedings Volume 7380, 73801V (2009) https://doi.org/10.1117/12.828314

KEYWORDS: Tumors, Photodynamic therapy, Bladder, Luminescence, Bladder cancer, Control systems, Animal model studies, Fluorescence correlation spectroscopy, Tissues, Green fluorescent protein

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Proceedings Article | 25 April 2008 Paper

Interest of second harmonic generation imaging to study collageneous matrix modification in osteoarthritis disease

Elisabeth Werkmeister, Natalia de Isla, Luc Marchal, Didier Mainard, Jean-François Stoltz, Dominique Dumas

Proceedings Volume 6995, 69950K (2008) https://doi.org/10.1117/12.781051

KEYWORDS: Collagen, Second-harmonic generation, Cartilage, Image compression, Fluorescence lifetime imaging, Luminescence, Image analysis, Molecules, Harmonic generation, Principal component analysis

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Cartilage degenerative diseases like osteoarthritis affect the organization of the biological extracellular matrix (ECM) surrounding chondrocytes. This ECM is mainly composed by collagen giving rise to a strong Second Harmonic Generation (SHG) Signal, due to its high non linear susceptibility. Mechanical stress leads to perturbation of the collagen network comparable to modification occurring in disease. To be sure that SHG signal comes specifically from the collagen network, the enzymatical action of Collagenase was followed. We clearly noted the decrease of the collagen specific signal according to incubation time due to enzymatic degradation. To characterize structural modification on the arrangement of collagen fibers in the ECM, we used image analysis based on co-occurrence matrix (Haralick). Textural features give information like homogeneity ('Angular Second Moment') or size of textural elements ('Inverse Difference Moment', 'Correlation'). Samples submitted to compression are characterized by higher 'Correlation', associated with a decrease of 'IDM' and 'ASM'. Those evolutions suggest the presence of long linear structures, an effect of packing of collagen fibrils and the apparition of nodes where the density of collagen is important versus areas showing a lack of molecules. Collagen I, II and VI are biomarkers characterising disease states since its presence is increased in pathological cartilage (osteoarthritis). Fluorescence Lifetime Imaging Microscopy (FLIM) associated to Spectral and SHG analysis confirmed the presence of Collagen I and II in the extracellular and Collagen VI in the pericellular matrix of chondrocytes. SHG, FLIM and Spectral Imaging combined with multiphoton excitation enable tissue imaging at deep penetration. We pointed out a local modification of the ECM of cartilage without any labelling (SHG) under mechanical stress. Thus the association of all these techniques represents a potential diagnosis tool for disorganization of collagen.

Proceedings Article | 14 April 2006 Paper

Study of polycation effects on erythrocyte agglutination mediated by anti-glycophorins using microscopic image digital analysis

B. Riquelme, D. Dumas, F. Relancio, A. Fontana, A. Alessi, P. Foresto, C. Grandfils, J. Stoltz, J. Valverde

Proceedings Volume 6191, 61911G (2006) https://doi.org/10.1117/12.662141

KEYWORDS: Image analysis, Blood, Digital imaging, Monoclonal antibodies, Luminescence, Manganese, Polymers, Camouflage, Statistical analysis, Confocal microscopy

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Proceedings Article | 8 October 2005 Paper

In vitro characterization of gap junctional intercellular communication by gap-FRAP technique

M. Abbaci, J.-R. Stines, M. Barberi-Heyob, W. Blondel, D. Dumas, F. Guillemin, J. Didelon

Proceedings Volume 5859, 585909 (2005) https://doi.org/10.1117/12.632829

KEYWORDS: Luminescence, Cancer, In vitro testing, Proteins, Head, Neck, Confocal microscopy, Microscopes, Tissues, Monoclonal antibodies

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Proceedings Article | 1 September 2004 Paper

Discrimination between normal and cancerous cells by gap-FRAP: feasibility in endoscopy

Jean-Rene Stines, Dominique Dumas, Walter Blondel, Jacques Didelon, Francois Guillemin

Proceedings Volume 5459, (2004) https://doi.org/10.1117/12.545495

KEYWORDS: Luminescence, Microscopes, Endoscopy, CCD cameras, Confocal microscopy, Cancer, Microscopy, Molecules, Tissues, Tissue optics

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Proceedings Article | 9 October 2003 Paper

Analysis of the 3D structure og agglutinated erythrocyte using CellScan and confocal microscopy: characterization by FLIM-FRET

Bibiana Riquelme, Dominique Dumas, Juana Valverde de Rasia, Rodolfo Rasia, Jean Francois Stoltz

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.500123

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Monoclonal antibodies, Confocal microscopy, Blood, Fluorescence lifetime imaging, Microscopes, Receptors, Molecules, Mathematical modeling

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Proceedings Article | 17 June 2002 Paper

Permeability oxygen through endothelial cells monolayer using time-resolved fluorescence microscopy: effect of human plasma viscosity

Dominique Dumas, M. Viriot, C. Boura, C. Gigant, V. Latger-Cannard, Jean Stoltz

Proceedings Volume 4620, (2002) https://doi.org/10.1117/12.491287

KEYWORDS: Plasma, Oxygen, Sensors, Luminescence, Diffusion, Hypoxia, Molecules, Microscopy, Time resolved spectroscopy, Capillaries

Showing 5 of 7 publications

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