There are summarized the data of author's investigations which demonstrate in the first time a native fluorescence of specimens of all classes of steroid hormones. Fluorescence spectra are described. The relative quantum yields of fluorescence are measured. The dependence of fluorescence intensity and spectral characteristics on the presence and the position of side functional groups and double bonds in hormone molecule are ascertained.
Kinetics of oil water solutions photodegradation under action of narrow band irradiation of XeBr*-excilamp (λ~283 nm) and iodine lamp (λ~206 nm) is presented. Power of lamps reached 10 mW/cm2. In all experiments TiO2 (rutile) powder was used as a catalytic agent. Photocatalytic degradation kinetics was controlled by laser fluorescence spectrometer. The measuring complex was based on the pulse-repetitive Nd:YAG laser (266 nm) with Q-switching. Dependencies of fluorescence intensity on irradiation time were made using laser-induced fluorescence spectra. It has been found that at initial time of irradiation, the photodegradation rate in oil aqueous solutions was higher in the case of XeBr* lamp, however, after 20 minutes of irradiation, major photodegradation was observed in the both cases.
The laser-induced fluorescence spectra of steroid hormones - estradiol, estriol, estrone, androstenedione - are obtained at excitation of the fourth harmonic of Nd:YAG laser radiation. The quantum yields of fluorescence of these substances were rated by means of the relative method. They are 1.11 X 10-1, 5.20 X 10-3, 8.47 X 10-5. The water solution of tryptophan was used as a standard. The set-up sensitivity for high and average quantum yields substances has been defined.
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