We present a CNN-based quantification pipeline for the imaging and analysis of adherent cell cultures. The imaging part features two CNNs dedicated to lens-free microscopy performing accelerated holographic reconstruction and phase unwrapping. The analysis part features CNNs estimating several cellular metrics. These CNNs maps phase image into 2D quantitative representations of cell positions and measurements. The outputs images are processed by a local maxima algorithm to obtain a list of cell measurements. Here, we discuss the performance and limitations of this CNN-based quantification pipeline. The advantage is the fast processing time, i.e. the analysis of ~10.000 cells in 10 seconds.
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