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Stain-free virtual histology of Bombus terrestris compound eyes by laboratory phase-contrast nano-CT
We have developed the tools necessary to simulate highly realistic small-animal XFCT. Our MC tool, XRFGPU is based on the accelerated open-source MC-GPU and allows simulations with speeds >1000x faster than other existing MC codes capable of similar simulations. Using available high-resolution digital mouse phantoms, we can predict the performance of any XFCT imaging arrangement and produce the realistic data needed for future development of small-animal XFCT. Here we validate the simulations against our laboratory arrangement, capable of 200 μm spatial-resolution XFCT of mice. Comparisons using a phantom confirms that the simulations are accurate. Lastly, we compare simulated images with images acquired in vivo using our laboratory arrangement showing how simulations can be used to enhance the interpretation of experimental data.
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