Dr. Hector Li-Chang Profile

Dr. Hector Li-Chang

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Proceedings Article | 21 February 2019 Paper

Enhanced spectral lightfield fusion microscopy via deep computational optics for whole-slide pathology

Farnoud Kazemzadeh, Hector Li-Chang, Alexander Wong

Proceedings Volume 10883, 1088312 (2019) https://doi.org/10.1117/12.2510952

KEYWORDS: Microscopy, Computer programming, Pathology, Interferometry, Computational imaging, Digital signal processing

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Lensfree on-chip microscopy, which harnesses holography principles to capture interferometric light-field encodings without the need of lenses, is an emerging microscopy modality with widespread interest given the large field-of-view (FOV) compared to lens-based microscopy systems. In particular, there is a growing interest on the development of high-quality lensfree on-chip color microscopy. In this study, we propose a multi-laser spectral lightfield fusion microscopy using deep computational optics for achieving lensfree on-chip color microscopy. We will demonstrate that leveraging deep computational optics can enable imaging resolution beyond the diffraction limit without the use of any complex hardware-based super-resolution techniques, such as aperture scanning. The capabilities of the microscope are examined for whole-slide pathology. The superior imaging resolution of the instrument is demonstrated by imaging of a series of biological specimens demonstrating the true color imaging capability of the instrument while showcasing the large FOV of the instrument.

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