Dr. Helmut W. Thissen Profile

Dr. Helmut W. Thissen

Principal Research Scientist at CSIRO

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Author

Publications (19)

Proceedings Article | 24 December 2011 Paper

Controlling cell-material interactions using coatings with advanced polymer architectures

Peter Koegler, Paul Pasic, Graham Johnson, Penny Bean, Guenter Lorenz, Laurence Meagher, Helmut Thissen

Proceedings Volume 8204, 82043T (2011) https://doi.org/10.1117/12.910456

KEYWORDS: Polymers, Polymerization, Plasma, Fluorine, Biomedical optics, Natural surfaces, Adhesives, Proteins, Computer architecture, In vivo imaging

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Proceedings Article | 30 December 2008 Paper

Magnetic and fluorescence-encoded polystyrene microparticles for cell separation

Diana Bradbury, Emily Anglin, Sheree Bailey, Peter Macardle, Michael Fenech, Helmut Thissen, Nicolas Voelcker

Proceedings Volume 7267, 726711 (2008) https://doi.org/10.1117/12.812208

KEYWORDS: Particles, Proteins, Magnetism, Luminescence, Nanoparticles, Tissues, Iron, Oxides, Microscopy, Quantum dots

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Proceedings Article | 30 December 2008 Paper

Preparation of chemical gradients on porous silicon by a dip coating method

Lauren Clements, Ljiljana Puskar, Mark Tobin, Frances Harding, Helmut Thissen, Nicolas Voelcker

Proceedings Volume 7267, 72670Q (2008) https://doi.org/10.1117/12.810718

KEYWORDS: Silicon, Infrared spectroscopy, Infrared microscopy, Coating, Chemistry, Ozone, Chemical analysis, Microscopy, Synchrotrons, Infrared radiation

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Proceedings Article | 27 December 2007 Paper

Enhancement of reverse transfection efficiency by combining stimulated DNA surface desorption and electroporation

Rhiannon Creasey, Andrew Hook, Helmut Thissen, Nicolas Voelcker

Proceedings Volume 6799, 67991D (2007) https://doi.org/10.1117/12.759382

KEYWORDS: Luminescence, Green fluorescent protein, Optical filters, Linear filtering, Scanners, Silicon, Glasses, Solids, Microscopy, Plasma

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Transfection cell microarrays (TCMs) are a high-throughput, miniaturised cell-culture system utilising reverse transfection, in which cells are seeded onto a DNA array resulting in localised regions of transfected cells. TCMs are useful for the analysis of gene expression, and can be used to identify genes involved in many cellular processes. This is of significant interest in fields such as tissue engineering, diagnostic screening, and drug testing^{[1, 2]}. Low transfection efficiency has so far limited the application and utility of this technique. Recently, the transfection efficiency of TCMs was improved by an application of a high voltage for a short period of time to the DNA array resulting in the electroporation of cells attached to the surface^{[3, 4]}. Furthermore, application of a low voltage for a longer period of time to the DNA array was shown to improve the transfection efficiency by stimulating the desorption of attached DNA, increasing the concentration of DNA available for cellular uptake^[5]. In the present study, the optimisation of the uptake of adsorbed DNA vectors by adherent cells, utilising a voltage bias without compromising cell viability was investigated. This was achieved by depositing negatively charged DNA plasmids onto a positively charged allylamine plasma polymer (ALAPP) layer deposited on highly doped p-type silicon wafers either using a pipettor or a microarray contact printer. Surface-dependant human embryonic kidney (HEK 293 line) cells were cultured onto the DNA vector loaded ALAPP spots and the plasmid transfection events were detected by fluorescence microscopy. Cell viability assays, including fluorescein diacetate (FDA) / Hoechst DNA labelling, were carried out to determine the number of live adherent cells before and after application of a voltage. A protocol was developed to screen for voltage biases and exposure times in order to optimise transfection efficiency and cell viability. Cross-contamination between the microarray spots carrying different DNA vectors was also investigated. By application of a voltage of 286 V/cm for 10 ms, transfection efficiency was doubled compared to using only transfection reagent, whilst maintaining a cell viability of 60-70% of the positive control.

Proceedings Article | 27 December 2007 Paper

Preparation of two-directional gradient surfaces for the analysis of cell-surface interactions

Lauren Clements, Yit-Lung Khung, Helmut Thissen, Nicolas Voelcker

Proceedings Volume 6799, 67990W (2007) https://doi.org/10.1117/12.759351

KEYWORDS: Silicon, Polymers, Electrodes, Plasma, Scanning electron microscopy, Chemistry, Atomic force microscopy, Etching, Glasses, Coating

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Proceedings Article | 9 January 2007 Paper

Microarrays for the evaluation of cell-biomaterial surface interactions

H. Thissen, G. Johnson, G. McFarland, B. Verbiest, T. Gengenbach, N. Voelcker

Proceedings Volume 6413, 64130B (2007) https://doi.org/10.1117/12.696030

KEYWORDS: Polymers, Coating, Glasses, Luminescence, Printing, Plasma, In vitro testing, Polymerization, Microscopy, Chemistry

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Proceedings Article | 9 January 2007 Paper

Application of nanostructured biochips for efficient cell transfection microarrays

Yamini Akkamsetty, Andrew Hook, Helmut Thissen, Jason Hayes, Nicolas Voelcker

Proceedings Volume 6413, 64130R (2007) https://doi.org/10.1117/12.695954

KEYWORDS: Silicon, Image filtering, Luminescence, Printing, Plasma, Linear filtering, Electrodes, Polymerization, Laser ablation, Proteins

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Proceedings Article | 9 January 2007 Paper

A novel surface modification approach for protein and cell microarrays

Mahaveer Kurkuri, Chantelle Driever, Helmut Thissen, Nicholas Voelcker

Proceedings Volume 6413, 64130Z (2007) https://doi.org/10.1117/12.715172

KEYWORDS: Proteins, Polymers, Glasses, Printing, Humidity, Plasma, Luminescence, Silicon, Solids, Ellipsometry

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Proceedings Article | 9 January 2007 Paper

A platform for the advanced spatial and temporal control of biomolecules

Andrew Hook, Helmut Thissen, Jason Hayes, Nicolas Voelcker

Proceedings Volume 6413, 64130C (2007) https://doi.org/10.1117/12.695595

KEYWORDS: Adsorption, Silicon, Plasma, Laser ablation, Luminescence, Polymerization, Polymers, FT-IR spectroscopy, Control systems, Microscopy

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Proceedings Article | 14 December 2006 Paper

Switchable surface coatings for control over protein adsorption

Martin Cole, Marek Jasieniak, Nicolas Voelcker, Helmut Thissen, Roger Horn, Hans Griesser

Proceedings Volume 6416, 641606 (2006) https://doi.org/10.1117/12.696290

KEYWORDS: Proteins, Adsorption, Silicon, Polymers, Ions, Polymerization, Statistical analysis, Interfaces, Semiconducting wafers, Biomedical optics

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Proceedings Article | 19 January 2006 Paper

Characterisation of porous silicon/poly(L-lactide) composites prepared using surface initiated ring opening polymerisation

Steven McInnes, Helmut Thissen, Namita Choudhury, Nicolas Voelcker

Proceedings Volume 6036, 60361X (2006) https://doi.org/10.1117/12.659973

KEYWORDS: Silicon, Polymerization, Polymers, Composites, Semiconducting wafers, Atomic force microscopy, Tin, Glasses, Tissue engineering, Infrared spectroscopy

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Proceedings Article | 16 February 2005 Paper

Switchable coatings for biomedical applications

Martin Cole, Nicolas Voelcker, Helmut Thissen

Proceedings Volume 5651, (2005) https://doi.org/10.1117/12.582306

KEYWORDS: Proteins, Adsorption, Plasma, Silicon, Polymers, Biomedical optics, Polymer thin films, Atomic force microscopy, Semiconducting wafers, Natural surfaces

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Proceedings Article | 16 February 2005 Paper

Development of an electro-responsive platform for the controlled transfection of mammalian cells

Andrew Hook, Helmut Thissen, Jason Hayes, Nicolas Voelcker

Proceedings Volume 5651, (2005) https://doi.org/10.1117/12.606789

KEYWORDS: Adsorption, Silicon, Plasma, Polymerization, Polymers, Excimer lasers, Luminescence, Laser ablation, Solids, Oxides

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Proceedings Article | 29 March 2004 Paper

Protein patterning in polycarbonate microfluidic channels

David Thomson, Jason Hayes, Helmut Thissen

Proceedings Volume 5275, (2004) https://doi.org/10.1117/12.524675

KEYWORDS: Proteins, Polymers, Microfluidics, Optical lithography, Plasma, Excimer lasers, Adsorption, Coating, Laser ablation, Diffusion

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Proceedings Article | 14 November 2002 Paper

Microarray platform for the creation of a matrix of site-specific transformed cells

Endre Szili, Helmut Thissen, Jason Hayes, Joe Shapter, Nicolas Voelcker

Proceedings Volume 4937, (2002) https://doi.org/10.1117/12.468658

KEYWORDS: Silicon, Plasma, Polymers, Proteins, Gold, Solids, Polymerization, Semiconducting wafers, Green fluorescent protein, Nitrogen

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Proceedings Article | 14 November 2002 Paper

Spatially controlled surface chemistry by excimer laser ablation of thin films

Helmut Thissen, Jason Hayes, Ben Muir, Micah Atkin, Erol Harvey

Proceedings Volume 4937, (2002) https://doi.org/10.1117/12.469160

KEYWORDS: Polymers, Plasma, Laser ablation, Chemistry, Excimer lasers, Silicon, Polymer thin films, Photomasks, Polymerization, Thin films

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Proceedings Article | 19 November 2001 Paper

Excimer laser ablation for spatially controlled protein patterns

Helmut Thissen, Jason Hayes, Peter Kingshott, Graham Johnson, Erol Harvey, Hans Griesser

Proceedings Volume 4590, (2001) https://doi.org/10.1117/12.454588

KEYWORDS: Proteins, Polymers, Plasma, Laser ablation, Adsorption, Excimer lasers, Collagen, Chemistry, Confocal microscopy, Oxides

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Proceedings Article | 19 November 2001 Paper

Fabrication and characterization of nanoscale biological coatings on synthetic carriers

Hans Griesser, Patrick Hartley, Sally McArthur, Keith McLean, Laurence Meagher, Helmut Thissen

Proceedings Volume 4590, (2001) https://doi.org/10.1117/12.454587

KEYWORDS: Proteins, Polymers, Plasma, Adsorption, Molecules, Silica, Atomic force microscopy, Molecular interactions, Biosensors, Solids

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Proceedings Article | 6 April 2001 Paper

Excimer laser ablation of plasma polymers for cell and tissue culture applications

Helmut Thissen, Jason Hayes, Patrick Hartley, Graham Johnson, Erol Harvey, Hans Griesser

Proceedings Volume 4234, (2001) https://doi.org/10.1117/12.424405

KEYWORDS: Polymers, Plasma, Laser ablation, Excimer lasers, Atomic force microscopy, Silicon, Chemistry, Coating, Fluorine, Nitrogen

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Showing 5 of 19 publications

Proceedings Volume Editor (1)

SPIE Conference Volume | 19 December 2008

Smart Materials V

Conference Committee Involvement (2)

Smart Materials V

10 December 2008 | Melbourne, Australia

Smart Materials IV

11 December 2006 | Adelaide, Australia

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