Autofluorescence endoscopy is a promising modality for diagnosis of colonic tumors. This article discusses the
origin of autofluorescence of the normal colon. Excised normal colons were analyzed by using fluorescence
stereomicroscopy and a fluorescence-lifetime microscopy system. Fluorescence images showed that the mucosa had
stronger autofluorescence than the submucosa. The results of fluorescence-lifetime measurement showed that
nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) might be responsible for the
autofluorescence of the colonic epithelia. Our results suggest that the mucosal autofluorescence generates by NADH and
FAD was an important source of the green autofluorescence.
We report the application of Optical coherence tomography (OCT) for visualizing a one dimensional depth resolved functional structure of cat brain in vivo. The OCT system is based on the known fact that neural activation induces structural changes such as capillary dilation and cellular swelling. Detecting these changes as an amplitude change of the scattered light, an OCT signal reflecting neural activity i.e., fOCT (functional OCT) could be obtained. Experiments have been done to obtain a depth resolved stimulus-specific profile of activation in cat visual cortex. Our results in one dimension indicate that indeed an orientation dependent functional signal could be obtained. Further, we show that this depth resolved fOCT signal is well correlated with the stimulus dependent column determined by OISI. Based on the results, the smallest functional unit in depth, resolved by the proposed system is around 40 micrometers . We are extending our system to perform two dimensional functional imaging.
A unique percutaneous transluminal coronary angioplasty system new IR therapy laser with IR glass fiber delivery under novel angioscope guidance was described. Carbon monoxide (CO) laser emission of 5 mm in wavelength was employed as therapy laser to achieve precise ablation of atheromatous plaque with a flexible As-S IR glass fiber for laser delivery. We developed the first medical CO laser as well as As-S IR glass fiber cable. We also developed 5.5 Fr. thin angioscope catheter with complete directional manipulatability at its tip. The system control unit could manage to prevent failure irradiations and fiber damages. This novel angioplasty system was evaluated by a stenosis model of mongrel dogs. We demonstrated the usefulness of our system to overcome current issues on laser angioplasty using multifiber catheter with over-the-guidewire system.
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