We report on the multiple wavelengths Raman laser output, the laser wavelength span from violet to mid-infrared, 359nm~9.2μm have been detected and recorded on spectrum and energy meter. In this investigation, 222mJ Q-switch Nd:YAG 1064nm laser set as pump laser, 737/770nm and 1.1/1.7/1.9/2.1/2.4/9.2μm Raman laser is high gained and takes a measure of the energy. 1.9μm laser maximum 28mJ, 2.1μm laser maximum 30mJ, laser pulse width is 4ns. And 9.2μm mid-infrared laser gets 0.8mJ. The pressurized hydrogen is pumped by a 1064nm laser and 737/770nm anti-Stokes Raman laser are generated. The 737nm laser is generated by stimulated vibrational Raman Scattering (SVRS); while the 770nm laser is generated by the combination processes of SVRS and stimulated rotational Raman scattering (SRRS). The maximum pulse energies of 6.42mJ at 737nm and 4.42mJ at 770nm are achieved by the optimization of anti-Stokes Raman laser. The energy efficiency is 2.6% at 737nm and 1.8% at 770nm.
In this paper, the laser confocal Raman spectrum of glucose standard solution was measured and studied. The relative intensity of the characteristic peak of glucose and the characteristic peak of water in the solution was used as the ratio of the measured content to measure the concentration of glucose solution.Put the prepared glucose solutions of different concentrations in a cuvette, place them on a glass slide, and place them under the field of view of the objective lens of the laser microscope Raman spectrometer stage, adjust the focal length through the eyepiece, focus the laser on the surface of the solution, and then measure Raman spectra of different solutions.The experimental results show that the relative intensity of the 1125cm-1 peak has the same trend as the actual value of glucose concentration, and there is a good linear relationship between them. This linear correlation reaches more than 0.93, and the measured LOD value is 160mg/dL This result confirms that the glucose concentration detected by Raman spectroscopy using this analytical method can be close to the normal value of human blood sugar. This will help to explore the relationship between the ratio of blood glucose peak to hemoglobin peak intensity and blood glucose content, and lay a good foundation for noninvasive blood glucose research.
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