Prof. Irene E. Kochevar Profile

Prof. Irene E. Kochevar

at Massachusetts General Hospital

SPIE Involvement:

Author

Publications (7)

SPIE Journal Paper | 20 September 2017 Open Access

Variations in the endogenous fluorescence of rabbit corneas after mechanical property alterations

Antonio Ortega-Martinez, Genna Touchette, Hong Zhu, Irene Kochevar, Walfre Franco

JBO, Vol. 22, Issue 09, 095005, (September 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.9.095005

KEYWORDS: Luminescence, Cornea, Molecules, Collagen, Eye, Ultraviolet radiation, Visualization, Tissues, Eye models, Statistical analysis

Read Abstract +

DOWNLOAD PAPER

Proceedings Article | 6 March 2015 Paper

Fluorescence spectroscopy of collagen crosslinking: non-invasive and in-situ evaluation of corneal stiffness

Walfre Franco, Antonio Ortega-Martinez, Hong Zhu, Ruisheng Wang, Irene Kochevar

Proceedings Volume 9327, 93270R (2015) https://doi.org/10.1117/12.2083702

KEYWORDS: Luminescence, Cornea, Collagen, Tissues, Eye, Fluorescence spectroscopy, Monochromators, Matrices, Proteins, Visualization

Read Abstract +

Proceedings Article | 25 April 2005 Paper

Photochemical bonding of epithelial cell-seeded collagen lattice to rat muscle layer for esophageal tissue engineering: a pilot study

Barbara Chan, M. Sato, Joseph Vacanti, Irene Kochevar, Robert Redmond

Proceedings Volume 5686, (2005) https://doi.org/10.1117/12.597181

KEYWORDS: Collagen, Tissues, Silicon, Tissue engineering, Argon ion lasers, Interfaces, Tissue optics, Structural engineering, Esophagus, Beam controllers

Read Abstract +

Bilayered tube structures consist of epithelial cell-seeded collagen lattice and muscle layer have been fabricated for esophageal tissue engineering. Good adhesion between layers in order to facilitate cell infiltration and neovascularization in the collagen lattice is required. Previous efforts include using other bioglues such as fibrin glue and silicone tube as the physical support. However, the former is subjected to chances of transmitting blood-born infectious disease and is time consuming while the latter requires a second surgical procedure. The current project aimed to bond the cell-seeded collagen lattice to muscle layer using photochemical bonding, which has previously been demonstrated a rapid and non-thermal procedure in bonding collagenous tissues. Rat esophageal epithelial cells were seeded on collagen lattice and together with the latissimus dorsi muscle layer, were exposed to a photosensitizer rose Bengal at the bonding surface. An argon laser was used to irradiate the approximated layers. Bonding strength was measured during the peeling test of the collagen layer from the muscle layer. Post-bonding cell viability was assessed using a modified NADH-diaphorase microassay. A pilot in vivo study was conducted by directly bonding the cell-seeded collagen layer onto the muscle flap in rats and the structures were characterized histologically. Photochemical bonding was found to significantly increase the adherence at the bonding interface without compromising the cell viability. This indicates the feasibility of using the technique to fabricate multi-layered structures in the presence of living cells. The pilot animal study demonstrated integration of the collagen lattice with the muscle layer at the bonding interface although the subsequent surgical manipulation disturbed the integration at some region. This means that an additional procedure removing the tube could be avoided if the approximation and thus the bonding are optimized. Cell infiltration and neovascularization were also evident demonstrating that direct bonding of engineered tissue structures in particular those with low processability such as collagen lattice to the host tissue is feasible.

Proceedings Article | 29 April 1998 Paper

Two-photon fluorescence and confocal reflected light imaging of thick tissue structures

Ki Kim, Peter So, Irene Kochevar, Barry Masters, Enrico Gratton

Proceedings Volume 3260, (1998) https://doi.org/10.1117/12.307081

KEYWORDS: Confocal microscopy, Luminescence, Tissues, Tissue optics, Microscopes, Skin, Photons, Chromophores, Objectives, Optical spheres

Read Abstract +

Proceedings Article | 7 August 1992 Paper

Upper excited-state photochemistry of DNA and nucleotides

Ivo Gut, Irene Kochevar

Proceedings Volume 1646, (1992) https://doi.org/10.1117/12.137460

KEYWORDS: Absorption, Ultraviolet radiation, Photons, Information operations, Picosecond phenomena, Quantum efficiency, Photochemistry, Visible radiation, Laser tissue interaction, Calorimetry

Read Abstract +

Proceedings Article | 1 May 1991 Paper

DNA photoproducts formed using high-intensity 532 nm laser radiation

Irene Kochevar, Yaron Hefetz, D. Dunn, T. F. Deutsch, Lisa Buckley, Franz Hillenkamp

Proceedings Volume 1403, (1991) https://doi.org/10.1117/12.57311

KEYWORDS: Absorption, Molecules, Photons, Laser applications, Visible radiation, Pulsed laser operation, Plasma, Molecular lasers, Photochemistry, Luminescence

Read Abstract +

Proceedings Article | 1 July 1990 Paper

Fluorescent-tip optical fiber probe for quantitative light dosimetry in light scattering media and in tissue

Lothar Lilge, Thomas Flotte, Irene Kochevar, James Foley, Brian Wilson

Proceedings Volume 1203, (1990) https://doi.org/10.1117/12.17678

KEYWORDS: Tissue optics, Luminescence, Optical fibers, Scattering, Absorption, Photodynamic therapy, Light scattering, Laser scattering, Water, Polymethylmethacrylate

Read Abstract +

Showing 5 of 7 publications

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years