We propose a method to construct a three-dimensional volumetric representation of uterine tissue through the full thickness of the uterine wall using Optical Coherence Tomography (OCT). Uterine tissue blocks from human donors were cut into ~4 mm thin slices and imaged on both sides using a commercial OCT system with 2 mm imaging depth. Attenuation compensation, rigid registration, image blending techniques were used to combine the OCT mosaic volumes from each side of the tissue into a single volume. Initial results suggest this method yields a representative 3D view of uterine collagen fiber architecture through the full tissue thickness.
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