Liver cancer has one of the highest rates of human morbidity and mortality. However, in terms of pathology, liver cancer is traditionally clinically diagnosed based on observation of microscopic images of pathological liver sections. This paper investigates in vitro samples of rat models of bile duct carcinoma and presents a quantitative analysis method based on microscopic hyperspectral imaging technology to evaluate liver cancers at different stages. The example-based feature extraction method used in this paper mainly includes two algorithms: a morphological watershed algorithm is applied to find object and segment pathological components of pathological liver sections at different stages, and a support vector machine algorithm is implemented for liver tumor classification. Majority/minority analysis is utilized as the postclassification tool to eliminate small plaques from the preliminary classification results. Then, pseudocolor synthesis in RGB color space is used to produce the final results. The experimental results show that this method can effectively calculate the percent tumor areas in liver biopsies at different time points, that is, 3.338%, 11.952%, 15.125%, and 23.375% at 8, 12, 16, and 20 weeks, respectively. Notably, through tracking analysis, the processed results of 8-week images showed the possibility for early diagnosis of the liver tumor.
Serious liver fibrosis will develop into liver tumor. Therefore, prevention and early treatment of hepatocellular carcinoma are the focuses of the medical community. To automatically identify and analyze the degree of liver fibrosis, a more intuitive and convenient approach is proposed to segmentation of liver pathological slice images. This paper aims to use hyperspectral image processing technology to analyze the pathological sections of liver tissue cells. The method uses the spectral math for image preprocessing, and utilizes the superior classification ability of neural net (NN) and support vector machines (SVM) to identify the pathological images of liver tissue. On this basis, Majority/Minority Analysis (MMA) is as the post classified tool to weaken small plaques interference. At last the original image and the classification results are synthesized by RGB bands, and good analysis results can be obtained. The experimental results show that the presented method has great practical value in clinical diagnosis.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.