The low level power laser has been used in dental treatments aiming to improve tissue healing. An in vitro study was performed to analyze the laser influence on gingival fibroblast. A human gingival fibroblast culture (LMF) was produced in DME medium with 10% bovine fetal serum (BFS) cells (LMF) were allocated in Petri plates and cultured in different SFB concentrations (0%, 5% e 10%). After 48 hours the plates were divided in 9 groups: 3 control: 3 irradiated by 635 nm laser; and 3 irradiated by 780 nm laser. The cultured cells received 4 applications, in 12 hours intervals, with energy dosage of 2 joules for each plate, by means of a punctual technique. The growth curves showed that the growth levels were lower in low BFS concentrations. The irradiation with laser accelerated the growth rate in all groups. Additionally, the number of cells developed in low BFS concentration (5%) and irradiated was similar to the number of control cells developed in ideal conditions (10% BFS). There was no statistically significant differences between the effects of the two types of laser studied.
We previously described the influence of low-level laser therapy (LLLT) on the primary fibroblast ATCC CRL1471 CCD-19SK passage 7 in culture, metabolic changes and statistical significance absorption of 3[H]Hydroxyproline after 2 irradiations (12 hour intervals) with Ar:DYE Laser, 633 nm wavelength, output power 38 mw, spot size 3.5 cm, power density 4 mw/cm2, energy density plus or minus 2 J/cm2. The aim of this work is to investigate, by using the same procedure, the behavior of the confluence monolayer fibroblasts culture when a central scratch of 0.4 - 1 mm and 2 irradiations is performed, by means of the study of the colony formation, haptotaxis (direction) and chemotaxis- chemokinesis (movement). The results indicate that all these phenomena appear sooner in the LLLT cultures than in non-treated cultures. Thus we can confirm that LLLT induces fibroblast biological effects.
In order to investigate histological results produced by laser wavelengths 514 and 585 nm (Monoline Argon Laser and cw Dye Argon Laser), tests were carried out on a haemangioma situated on the neck of a male adult. The tests were done using the hexascan, previously modified to receive two fibers, which could separately direct the wavelengths 514 and 585 nm (1 watts and 30 ms pulses), with slight pauses during the treatment of one area and another, but both areas having identical characteristics. Random histological samples were taken previous to treatment, immediately after, and a month following treatment, processing them with Mason's Trichromic. Analysis and evaluation of the samples were performed by two researchers, strange to the investigation. In addition to the typical tissue reactions to relatively long Argon pulses (longer than 100 ms), it was found that there were no significant differences in the skin and vessels histological reactions, when both wavelengths 514 and 585 nm were used.
Since the start of Laser Medicine in the early 60's, there has been an increased interest in its application, throughout the world. Pelt's well4cnown expression when referring to laser: "a solution when looking for a problem" is as if he was revolted during research, as if he set to work feverishly in the search for practical application of characteristic laser effects. Due to laser characteristics, such as the brilliance, parallelism and monochromaticity, extensive practical medical expressions have found their place in therapy and diagnosis, which in the specialties of ophthalmology and neurosurgery, medical laser can be considered an essential instrument of great esteem.
Low power laser has been claimed, both at laboratory and
for clinical treatment to activate wound healing. Chronic ulcers
respond very positively to laser treatment when particular rules
of irradiation are take into account. The multiple etiology of
chronic ulcers is not conductive to treatment selection,
including laser treatment, if the associated illness is not taken
into consideration.
For more than 14 years our clinical experience have been
significantly positive using lasers in the treatment of chronic
ulcers. Our causistic, based on 242 cases treated from 1975
through 1983, has kept in many cases very close follow-up for an
extended time periods of up to six years after healing. By
controlling photographically and microscopically a chronic venous
ulcer submitted to low density laser irradiation, as well as by
studying the process of reparation of experimental ulcers and
burns, produce on laboratory animal, the healing effects of laser
radiation can be followed.
Statistically, it is possible to estimate that low
intensity laser irradiation produces faster reparation of damage
tissue.
Conference Committee Involvement (1)
Mechanisms for Low-Light Therapy
22 January 2006 | San Jose, California, United States
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