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Kangkang Liu

at Nanjing Univ

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Proceedings Article | 20 December 2019 Paper

An immunoturbidimetric assay for specific proteins identification from whole blood based on multi-layered centrifugal microfluidic chip

Jiachen Yang, Kangkang Liu, Minghao Yang, Guanghui Wang

Proceedings Volume 11209, 112094F (2019) https://doi.org/10.1117/12.2549593

KEYWORDS: Blood, Proteins, Microfluidics, Plasma, Calibration, Capillaries, Biological research, Liquids, Absorbance, Biomedical optics

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In this study, a portable and automatic immunoturbidimetric assay system based on multi-layered centrifugal microfluidics was developed for specific proteins identification from whole blood. With the development of society and medical technology, more and more immune system diseases have been noticed by people. Immune dysfunction in the human body can cause many immune system diseases. Therefore, the detection of specific proteins closely related to immune system diseases has been increasingly favored and paid more attention by medical and scientific researchers. However, for most automated biochemical analyzers, pretreatment and diagnosis process are separated, cannot provide a sample-to-answer solution. To solve this problem, we proposed a highly-integrated system which enables real-time sedimentation of blood cells by centrifugation and quantitative extraction of purified plasma by siphon valve. Also, it has calibration system of standard curves, as well as integrated optical detection device, leading to smaller error fluctuations. As a demonstration experiment, quantification of Immunoglobulin A (IgA) in human whole blood with our LOAD system was conducted. Calibrator sets with specified concentrations ranging from 0.6 to 6 g/L were first generated in real time on the chip. Then standard curves used for IgA quantitation were created. Our LOAD system was demonstrated to have good accuracy and high repeatability since the experimental results of IgA obtained from the LOAD system correlated very well with those from the standard automatic biochemical analyzer method. Furthermore, the LOAD system could conduct simultaneous detection of various specific proteins in the later stage.

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