Direct imaging of metabolism at sub-cellular resolution provides insights to understand single-cell physiology at spatially resolved manner. Here, we presented a metabolic profiling platform based on Optical Photothermal Infrared (OPTIR) spectroscopic imaging that enables in situ lipid metabolism characterization at sub-cellular resolution. We applied the developed metabolic imaging platform to study lipid synthesis from azide-labeled fatty acids across various systems including neuroglioma cells, human-induced pluripotent stem cells (iPSCs), iPSC-derived microglia cells, and iPSC-derived brain organoids. The specificity of the developed platform was further improved by coupling a fluorescence module that enables cell type specific-metabolic profiling. Collectivity, the proposed metabolic imaging platform opens a vast potential for in situ metabolism characterization at the single-cell level.
We developed an optical infrared photothermal (OPTIR) metabolic imaging platform that enables in situ lipid metabolism characterization at sub-cellular resolution. The cell-type specific lipid metabolic imaging was achieved through the fluorescence module integrated into the OPTIR system. We successfully imaged newly-synthesized lipids after azide-palmitic acid treatment in cells across different model systems including neuroglioma, human-induced pluripotent stem cells (iPSCs), iPSC-derived microglia cells, and iPSC-derived brain organoids. The metabolic heterogeneity in different cell types in brain organoids was observed. Collectivity, the proposed metabolic imaging platform opens a vast potential for in situ metabolism characterization at the single-cell level.
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