Various algorithms have been proposed to compute attenuation coefficient (AC) based on optical coherence tomography (OCT) signals. The single scattering model has gained widespread application due to its computational efficiency; however, it’s only applicable to thin tissue layers, thus imposing strict requirements on the data length used for calculations. This study compared the algorithms for estimating the AC from OCT data using different data length such as 180 μm , 250 μm , 350 μm , 435 μm acquired from intralipid phantoms. Subsequently, employing various algorithms and utilizing the optimal data length, we calculated the attenuation coefficients of liquid phantoms with different concentrations, and the methodology was applied to homogeneous mouse tissues. The comparative study may provide a reliable basis for parameter configuration for AC extraction algorithm.
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