This study investigates an indirect measurement of the antibiotic susceptibility of Escherichia coli (E. coli) strain ATCC 25922 by analyzing sample background fluorescence at 455 nm excitation and 530 nm emission wavelengths. The study takes a practical approach by exposing the bacterial culture, suspended in Luria-Bertani (LB) broth medium, to various concentrations of ampicillin and analyzing the corresponding fluorescence intensities of the inhibitory and non-inhibitory ranges of antibiotic concentrations. The variations in fluorescence intensities indicate probable antibiotic sensitivity. The results are validated with gold standards such as the broth microdilution method.
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