In the life sciences, mechanical properties of cells can be used as a diagnostic tool for breast cancer or liver fibrosis, but current techniques require long integration times. The promising impulsive stimulated Brillouin scattering microscopy is an all-optical approach allowing for both a non-invasive and high-resolution measurement. The study reveals that maximizing pulse energy and pulse width achieves high SNR and fast image acquisition, but caution is necessary to stay below the phototoxicity threshold. Imaging at a 10 kHz repetition rate with 20 averages yields good SNR and an imaging speed of 2 ms/pixel, with potential for improvement. A new technique using asymmetric pump focus shows promise in increasing the lateral resolution.
Impulsive stimulated Brillouin microscopy (ISBS) is a technique that promises to enable fast measurements of the viscoelastic properties of biological samples. Its big advantage compared to spontaneous Brillouin arises from the capability to manipulate the signal-to-noise ratio by the choice of the system parameters. In this contribution, we will present a thorough analysis of the signal generation dependencies and the resulting implications on the spatiotemporal resolution. First measurements on blood, water and hydrogels underline the potential of the technique and show that ISBS can be an important alternative for selected biomedical applications.
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