A microfluidic single molecule fluorescence-based detection scheme is developed to identify target protein direct from
cell lysate by using polyclonal antibody. Relative concentration of target protein in solution is determined by twodimensional
(2D) photon burst analysis. Compared to conventional ensemble measurement assays, this microfluidic
single molecule approach combines the advantages of higher sensitivity, fast processing time, small sample consumption
and high resolution quantitative analysis.
The Raman scattering signature of molecules has been demonstrated to be greatly enhanced, on the order of 106-1012
times, on roughened metal surfaces and clustered structures such as aggregated colloidal gold. Here we describe a
method that improves reproducibility and sensitivity of the substrate for surface enhanced Raman spectroscopy (SERS)
by using a nanofluidic trapping device. This nanofluidic device has a bottle neck shape composed of a microchannel
leading into a nano channel that causes size-dependent trapping of nanoparticles. The analyte and Au nanoparticles, 60
nm in diameter, in aqueous solution was pumped into the channel. The nanoparticles which were larger than the narrow
channel are trapped at the edge of the channel to render an enhancement of the Raman signal. We have demonstrated
that the Raman scattering signal enhancement on a nanochannel-based colloidal gold cluster is able to detect 10 pM of
adenine, the test analyte, without chemical modification. The efficiency and robustness of the device suggests potential
for single molecule detection and multicomponent detection for biological applications and/or biotoxins.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.