It has been known that the shape, the locomotion, and the growth of cells and bacteria are often affected by their
interactions with extra cellular matrix (ECM). However, it is difficult to quantify such interactions with conventional
biochemical methods. In this paper we report the application of oscillatory optical tweezers to trap and oscillate
three types of E. coli, in 0.2% LB agar substrate to quantify the E. coli - substrate interactions in terms of the
elasticity modulus G'. The three types of E. coli are BW25113 (wild-type, normal with flagellum), BW25113
(normal with flagellum, but subjected to UV light exposure for 1 hr to deactivate the flagellum), and JW1923 (a
null-flagellum mutant of BW25113). Our results indicate that the value of G' for the later two is significantly higher than
that for the normal wild-type (WT). We speculated that the interaction with the surrounding is perturbed, and hence
reduced, mainly by the motion of the flagellum in the case of the WT.
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