Atopic dermatitis (AD) and psoriasis are the two most prevalent skin disorders, often assessed through subjective questionnaires or visual evaluations conducted by clinicians, which can be subject to interpersonal variations. This study aims to explore the distinctions between these skin conditions and healthy skin using a portable confocal Raman spectroscopy (CRS) system for objective assessment. Spectral measurements at 671 nm and 785 nm on 9 AD, 6 psoriasis, and 11 healthy subjects reveal lower water content in AD compared to psoriasis and healthy skin. Ceramide subclasses show disease-specific trends, distinguishing AD, and psoriasis. Cholesterol levels further differentiate these conditions, with lower concentrations in lesional AD and significantly higher concentrations in lesional psoriasis compared to healthy skin. These differences contribute to the objective differentiation of skin conditions aiding in thorough assessment and treatment monitoring. Furthermore, it offers valuable insights for developing targeted disease-specific topical treatments.
This conference presentation was prepared for the Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics conference at SPIE BiOS, 2023.
Auretek is the first of its kind portable Confocal Raman Spectroscopy (CRS) system equipped with fiber based handheld probe, that cascades dual-wavelength lasers. With its flexible probe, Auretek can acquire CRS data of skin from various parts of the human body with depth profiling. A clinical study was conducted to investigate the effects of ceramide-based moisturizer (CBM) on skin physiology and biochemistry. Quantitative skin component characterization in different epidermal layers was done with spectral unmixing analysis of the CRS data. Even though the analysis revealed a significant increase in ceramide content on the side of CBM application, the increase was in the layers within the stratum corneum.
Confocal Raman spectroscopy (CRS) technology has wide applications, especially in the biomedical field. CRS provides non-destructive, contrast-free and molecule-specific quantitative measurement of samples, including biological tissues. These advantages make it ideal for human skin characterization. We present a portable dual-wavelength CRS system with a handheld measurement probe which connected to the console by optical fibers. To achieve fast switching between the two wavelengths, a fast 2-to-1 fiber array multiplexing module was used. A large bandwidth could be covered at each measurement point in real time. We performed system characterization for this proposed CRS system and measured different samples.
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