Ms. Rui Liu Profile

Rui Liu

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 15 March 2023 Poster + Paper

Feasibility study of Raman spectroscopy in corneal stromal tissue of human myopic eye

Ming Han, Jing Li, Nan Wang, Rui Liu, Yong Li, Juan Li, Qi Zeng, Xueli Chen

Proceedings Volume 12392, 123920D (2023) https://doi.org/10.1117/12.2655545

KEYWORDS: Raman spectroscopy, Principal component analysis, Cornea, Tissues, Eye, Proteins, Objectives, Confocal microscopy, Raman scattering, Nucleic acids

Read Abstract +

Myopia, a global public health problem, is recognized by the World Health Organization as the leading cause of visual impairment in uncorrected people. At present, a large number of reports focus on the pathological manifestations of retinal level myopia. However, corneal histological changes that may be associated with myopia have not been thoroughly investigated. Raman spectroscopy is a rapid and non-destructive analytical technique with the advantages including label-free, non-invasive and highly specific, providing detailed information at the molecular level. Important components of all the human tissue (proteins, nucleic acids, lipids, etc.) have corresponding Raman spectral characteristic peaks, which contribute to the study of myopia at the molecular level. In this study, a microscopic confocal Raman system (MCRS) was built to collect Raman spectrum of corneal stromal samples, which was obtained through femtosecond laser small incision corneal stroma lens extraction (SMLIE). One hundred fifty-nine corneal stromal Raman spectrum data were collected (54 low myopia, 69 moderate myopia and 36 high myopia). Ten characteristic peaks and corresponding components were further identified. K-nearest neighbor (KNN) was used with principal component analysis (PCA) to classify the samples and the classifications were validated by k-fold cross-validation. Three types of samples with different degrees of myopia were differentiated under the PCA-KNN model with an accuracy of 93.1%. Two characteristic peaks (1099 cm^-1 and 2940 cm^-1) show greatly contribution to the classification results. The results provide that Raman spectroscopy combined with PCA-KNN analysis can effectively distinguish the degree of myopia and is expected to explore the potential causes of myopia.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years