Mr. Scott J. Sottosanti Profile

Scott J. Sottosanti

at Dartmouth Hitchcock Medical Ctr

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Author

Publications (7)

Proceedings Article | 13 March 2024 Presentation

Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms

Logan Bateman, Valentin Demidov, Natalia Demidova, Jason Gunn, Scott Sottosanti, Eric Henderson, Ida Gitajn, Jonathan Elliot

Proceedings Volume PC12822, PC128220G (2024) https://doi.org/10.1117/12.3003128

KEYWORDS: Biofilms, Photodynamic therapy, Light sources, Biomass, Visible radiation, Titanium, Tissues, Surgery, Oxygen, Optical coherence tomography

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Proceedings Article | 13 March 2024 Presentation + Paper

Portable red-blue light source for antimicrobial photodynamic therapy of orthopaedic implant biofilms

V. Demidov, J. Sottosanti, N. Demidova, L. Bateman, O. Jackson, P. Craig, D. Hazem, J. Gunn, I. Gitajn, J. Elliott

Proceedings Volume 12822, 1282208 (2024) https://doi.org/10.1117/12.3000416

KEYWORDS: Biofilms, Light sources, Optical coherence tomography, Bioluminescence, Light emitting diodes, Microfluidics, Titanium, Photodynamic therapy, Contamination, Tissues

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Well-organized ecosystems of bacteria colonize orthopaedic devices causing biofilm infections that are notoriously difficult to manage. Biofilms typically exhibit increased resistance to antibiotics leading to treatment failure, and tools for eradicating biofilms that do not increase antibiotic resistance are greatly needed. Antimicrobial photodynamic therapy (aPDT) is a promising form of treatment to combat clinically relevant biofilms. Exogenous provision of 5-aminolevulinic acid (5-ALA) to biofilm-forming clinical strains of E. coli, E. faecalis and S. aureus was recently shown by several research groups to result in the accumulation of sufficient quantities of endogenous photosensitizers porphyrins (protoporphyrin IX, coproporphyrin III and others), via the heme biosynthetic pathway, to produce a significant phototoxic effect when exposed to activating light. For clinical translation of this extremely promising approach, here we develop a portable light source for 5-ALA-based aPDT of orthopaedic implant biofilms, spectrally shaped for optimal porphyrin light absorption at wavelengths range approved by FDA for clinical use. After phantom calibration, we tested it on E.coli-E.faecalis biofilms grown in soft lithography-fabricated microfluidic chips and on methicillin-resistant S. aureus (MRSA) biofilms grown on titanium and stainless steel orthopaedic hardware in custom-designed macrofluidic devices. Successful in-vitro experiments allowed us to conduct a proof-of-concept validation study in a preclinical rat model of MRSA-contaminated open fracture. Following tibia fracture and two hours of wound infection development, a one hour incubation with 20% 5-ALA and treatment with either 90J/cm² or three fractions of 30J/cm² light doses demonstrated 94% and 99% overall reduction of MRSA, respectively, while the temperature of the tissue remained <39°C, below the threshold for thermal damage. The encouraging results suggest further preclinical testing of the developed light source for optimization of aPDT regimen and 5-ALA concentration to reduce the risk of long-term side effects in animal models of contaminated trauma surgery.

Proceedings Article | 13 March 2024 Poster + Paper

Development of a rabbit osseointegration prosthesis model to investigate biofilm infection and antimicrobial photodynamic therapy

Jason Gunn, Valentin Demidov, Olivia Jackson, J. Scott Sottosanti, Logan Bateman, Bailey Fearing, Joseph Hsu, Rachel Seymour, Jonathan Thomas Elliott, I. Leah Gitajn

Proceedings Volume 12822, 128220D (2024) https://doi.org/10.1117/12.3003089

KEYWORDS: Biofilms, Bioluminescence, Animals, Bacteria, Skin, Tissues, Optical coherence tomography, Biological imaging, Surgery, Bone

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In orthopedic trauma surgery, traditional socket-based prostheses are associated with functionally limiting problems affecting 1.7 million amputees in the United States. To improve post-surgical performance and minimize socket-related complications, bone-anchored (osseointegrated) prostheses have been developed. Functionally superior, their widespread implementation has been limited due to infection. In an unacceptable number of patients well-organized biofilm ecosystems of bacteria colonize the osseointegrated implant (OI) and migrate into device-tissue interface, leading to superficial and deep infections, and implant failure. Since the OI implant protrudes through the skin, the site is easily contaminated by microbes. The problem is worsened by increased resistance to antibiotics contributing significantly to surgical outcome failure. Antimicrobial photodynamic therapy (aPDT)—which uses photosensitizers excited with visible light to disrupt biofilms and kill bacteria with produced reactive oxygen species—has been proposed to address this problem. To assess biofilm formation and aPDT effectiveness, we describe a rabbit OI model and steps to investigate the ability of aPDT using 5-Aminolevulinic acid (5-ALA)-based light therapy to control methicillin-resistant S. aureus (MRSA) bacterial infection. As part of an institutionally approved survival surgery, this model involves lower limb amputation at the tibia, OI installation and MRSA inoculation. Within a week of biofilm formation, the optimal aPDT regime of light and 5-ALA dose was applied to the implant-skin interface to eradicate migrating biofilms. We have built a circumferential light source spectrally shaped for optimal photoactivation and cooled without risk of bacteria dispersal. Optical coherence tomography (skin flap healing and side-effects), micro-computed tomography (OI-bone integrity) and bioluminescence (bacterial bioburden before and after aPDT) imaging were used to monitor outcome for up to three weeks post-treatment.

Proceedings Article | 13 March 2024 Presentation + Paper

Quantification of methicillin-resistant S. aureus (MRSA) biofilm formation on orthopedic implants using bioluminescence imaging

Olivia Jackson, P. Lucas Craig, Dana Hazem, Natalia Demidova, Jason Gunn, J. Scott Sottosanti, Jonathan Thomas Elliott, Valentin Demidov, I. Leah Gitajn

Proceedings Volume 12822, 1282205 (2024) https://doi.org/10.1117/12.3001766

KEYWORDS: Bioluminescence, Biofilms, Bacteria, Titanium, Stainless steel, Standards development, In vitro testing, Microfluidics, Plating, In vivo imaging

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Proceedings Article | 15 March 2023 Presentation + Paper

Fluorescence-guided and molecularly guided debridement: identifying devitalized and infected tissue in orthopaedic trauma

Jonathan Thomas Elliott, Eric Henderson, Samuel Streeter, Valentin Demidov, Xinyue Han, Yue Tang, J. Scott Sottosanti, Logan Bateman, Petr Brůža, Shudong Jiang, I. Leah Gitajn

Proceedings Volume 12361, 1236108 (2023) https://doi.org/10.1117/12.2661243

KEYWORDS: Tissues, Bone, Surgery, Injuries, Imaging systems, Fluorescence, Biofilms, Fluorescence imaging, Fluorescence intensity, Blood circulation

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Proceedings Article | 5 March 2021 Presentation + Paper

Pulsed-light illumination optical system integrated into surgical microscope for 5-ALA-induced tumor fluorescence detection without surgical process interruption

Valentin Demidov, J. Scott Sottosanti, Dennis Wirth, Linton Evans, David Roberts, Keith Paulsen, Jonathan Elliott

Proceedings Volume 11625, 116250I (2021) https://doi.org/10.1117/12.2582839

KEYWORDS: Tumors, Microscopes, Fluorescence spectroscopy, Tissues, Brain, Tissue optics, Switches, Surgery, Navigation systems, Magnetic resonance imaging

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Proceedings Article | 5 March 2021 Paper

A portable polyanatomic optico-impedance system for early warning of systemic disease: evaluation in occult hemorrhage

Jonathan Elliott, J. Scott Sottosanti, Ethan Murphy, Samuel Klein, Vikrant Vaze, Ryan Halter, Norman Paradis

Proceedings Volume 11651, 116510N (2021) https://doi.org/10.1117/12.2583327

KEYWORDS: Spectroscopy, Pathophysiology, Near infrared spectroscopy, Machine learning, Light emitting diodes, Human subjects, Diagnostics, Blood, Abdomen

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Showing 5 of 7 publications

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