Mapping the distribution of chemical molecules throughout a brain is helpful for neuroscience research. We have applied an ultra-broadband multiplex CARS spectroscopic imaging system to construct a whole-brain label-free molecular map in macro and micro scales. We could precisely visualize lipids distributed to white matter, rich in neuronal fibers. Our microscale measurements figured out that cells within the hippocampus and cerebral cortex could be divided into lipid-rich and water-rich cells. Moreover, we applied multivariate curve decomposition analysis for our spectrum and recapitulated the results. Our imaging and analysis techniques will lead to the molecular brain atlas with single-cell resolution.
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