We present a cost-affordable capacitive aptasensor specific to Omicron variant of SARS-CoV-2. Laser-induced graphene (LIG) electrodes were used as transducer surfaces due to their performance and facile and low-cost production. Electrochemical impedance spectroscopy (EIS) was applied as signal transduction for biosensing studies of Omicron variant of SARS-CoV-2 and non-target viruses by aptasensor. At lower viral load, the aptasensor showed to be sensitive and specific for Omicron. At higher viral load, aptasensor is not able to differentiate between target and non-target viruses. The proposed biosensor showed promise for detecting Omicron variant of SARS-CoV-2 during early infection stage when concentration of intact virion is low.
The high transmission rate and fast mutation capacity of SARS-CoV-2 requires development of low-cost/widely deployable biosensor technologies. Here, we describe the development of an enzymatic biosensor to monitor SARS-CoV-2 and its emerging variants in human saliva based. Biotinylated angiotensin converting enzyme (ACE2) was immobilized on streptavidin-coated fiber optic probes and gold chips for biolayer interferometry (BLI) and surface plasmon resonance (SPR), respectively. Kinetics of ACE2-virion binding for wild-type, Delta, UK, and South Africa variants were analyzed and compared for informing the development of a mobile detection system.
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