We present the compact SECM system with a 3D-printed tethered endoscopic capsule with a 1060 nm center wavelength. Compared to the previously presented 1310 nm SECM system, the present SECM system theoretically provides 15% improved lateral resolution, 5.2 times faster imaging speed, and two times faster fabrication times. We performed in vivo imaging on the swine esophagus and the SECM images clearly visualized squamous cell nuclei as a bright dot surrounded by dark cytoplasm and bright cell borders. These results indicate that our new SECM endoscopic system making it easier to conduct cellular-level diagnosis of upper GI tract.
Dynamic micro-optical coherence tomography (DµOCT) is a technology that is capable of interrogating intracellular dynamics in intact, viable tissues. Towards our goal of advancing DµOCT for phenotyping cells, we imaged freshly excised human biopsies and performed correlative studies with histological results. To date, more than 30 biopsies from 17 patients with numerous types of gastrointestinal pathologies, including cancer, diverticulitis, and Crohn’s disease were imaged. In addition, using mouse models, we performed DµOCT imaging studies on tumors locally treated with chemotherapeutics delivered via custom implantable microdevices to observe the impact of those drugs on the tumor. Cyclical immunofluorescence staining was used to co-register ~20 markers on the same cross-sectional plane. We further demonstrate the utility of principal component analysis, K-means clustering, and convolutional long short-term memory (ConvLSTM) neural network for expanding the capabilities of DµOCT.
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