Uncooled micro-bolometer of 17 μm pitch has been successfully applied to the micro-scale thermal analysis with a
newly designed optics, the emissivity corrected procedure, and the quasi-acceleration of time frame method. The spatial
resolution of 11 μm in a good balance of MTF and N.A. has been achieved for the optics of VOx FPA. It enables to
visualize the exothermic latent heat of freezing biological cells at a minus temperature. The emissivity corrected microscale
thermal imaging using a real time direct impose-signal system is presented both with VOx and InSb FPA.
We report on a combined differential scanning calorimetric (DSC) and Raman scattering study of thermal
polymerization of sol-gel organic-inorganic SZ2080 and SU-8 resists. In SZ2080, endothermic peak at 95°C
signify drying of the resist and justifies the required pre-bake at around 100°C for 1-2 h for the best performance
during femtosecond (fs-)direct laser writing. A strong exothermic peak at 140°C (under 2 K/min heating rate)
completes polymerization of the resist. It is revealed that 1wt% of photoinitiators change Raman scattering
intensity of SZ2080 and can contribute efficiently to heating and cross-linking of photo-polymers. In the case
of SU-8, a 65°C DSC feature related to solvent evaporation was observed. The strongest changes in Raman
spectrum occurs at a narrow 895 cm-1 band which is linked to polymerization. Raman scattering taken during
DSC revealed spectral changes following the polymerization; an applicability of this method for monitoring photopolymerization
induced by ultra-fast laser sources and feasibility of a laser-modulated calorimetry is discussed.
Creating and monitoring micro-scale thermal transfer in micro-structured thin polymer films is presented in this study.
Thermal imaging is captured using IR camera equipped with optics designed for the mid- and long- IR wavelengths. The
non-contact thermal imaging method is preferred to visualize the distribution of temperature and characterizes the
thermal properties of complex multi-component/layered structures of thermal functioning materials. The work is to
present a method for thermal wave imaging that is applied to measure the thermal diffusivity of the micro-structured
polymer thin films using a modulated laser-diode spot heating irradiated to the rear surface of the film, observed at
different modulation frequencies. The procedure is based on the micro-scale thermography and the analysis is based on
the computational phase lock-in system for the temporal evolution extracted from the sequence of infrared image. The
in-plane surface profiles of the amplitude and phase are precisely calculated and the principle for eliminating the effects
of heat loss is examined.
The applications of uncooled micro-bolometer VOx FPA to the micro-scale thermal analysis of polymeric & organic
materials are presented. The latent heat during phase transition is analyzed with the emissivity correction calculation for
all pixels that include the calibration algorithm using a real time direct impose signal system. It enables to visualize the
exothermic latent heat of freezing biological cells at minus temperature. In comparison with the previously obtained data
by using the InSb FPA sensor, the limitation and the possibility of the un-cooled micro-bolometer in view of application
in thermal analysis of materials characterization are discussed.
We report on the application of two-dimensional micro-scale thermal analysis with a high-speed infrared (IR) camera
equipped with an originally designed optics for the optimum wavelength 3 - 5 micron in order to observe the exothermic
latent heat and thermal diffusion during the phase transitions of organic molecular crystals (n-alkanes; n- pentacosane, ntetracosane,
and n- tricosane) and polymeric spherulites of poly(ethylene oxide) (PEO). The forerunning phenomenon of
phase transition was visualized as the spatial temperature fluctuation by a two-dimensional differential calculus. The
exothermic heat patterns of the lamellae growth of n- alkanes and that of spherulites in the radial direction of PEO were
observed.
Two-dimensional micro scale thermal analysis is proposed for the measurement of the latent heat released from the cultured vascular endothelial cells during freezing by use of high-speed infrared focal plane arrays. The thermo graphically observed latent heat spreading over the tissues and the inter- or intracellular thermal diffusion were detected with a spatial resolution of 7.5mm. The temperature distribution in time and space in each endothelial cell was influenced by both the self-release and intercellular diffusion of latent heat. The effect of cryo-protective agent on the cell suspension of bovine fetal aorta endothelial cell (BFAEC) was examined by the observation of latent heat during the repeated freezing processes.
Using a high speed IR camera for temperature sensor is a powerful new tool for thermal analysis in the cell scale biomaterials. In this study, we propose a new type of two-dimensional thermal analysis by means of a high speed IR camera with a microscopic lens, and applied it to the analysis of freezing of plant and animal cells. The latent heat on the freezing of super cooled onion epidermal cell was randomly observed by a unit cell size, one by one, under a cooling rate of 80degC/min with a spatial resolution of 7.5m. The freezing front of ice formation and the thermal diffusion behavior of generated latent heat were analyzed. As a result it was possible to determine simultaneously the intercellular/intracellular temperature distribution, the growing speed of freezing front in a single cell, and the thermal diffusion in the freezing process of living tissue. A new measuring system presented here will be significant in a transient process of biomaterials. A newly developed temperature wave methods for the measurement of in-plane thermal diffusivity was also applied to the cell systems.
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