A new method based on self-assembly and transfer printing was presented for plasmonic coloration. It could be adopted for not only different rigid substrates but also flexible substrates no matter the substrate is hydrophilic or not so that solvent-response plasmonic pattern could be fabricated. This method is low-cost, stable, and surface-universal to realize large-area plasmonic coloration.
Multiplex assays have attracted considerable interest to meet the growing demand for clinical diagnosis, gene expression,
drug discovery, and so on. Most of the assays are based on molecular binding or recognition events. In this point,
different probe biomolecules could be immobilized to encoded carriers, which can be mixed and subjected to an assay
simultaneously and then many binding events can be distinguished by their codes. Herein we summarize our work on
photonic beads as novel encoded carriers of biomolecules in multiplex bioassays. We have successfully fabricated
different kinds of encoded photonic beads with controlled size and monodispersity by microfluidic device. The beads
with opal structure and inverse opal structure could be used in multiplex labeling detection and label-free detection of
biomolecules, respectively. These photonic beads provide a new coding strategy of suspension array for low cost,
sensitive and simultaneous multiplex bioassay.
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