Photo-mediated ultrasound therapy (PUT) is a novel, non-invasive, and agent-free therapeutic technique that uses a combination of relatively low-intensity ultrasound bursts and nanosecond laser pulses to selectively and precisely remove highly optically absorptive targets. In this work, we developed an integrated ultrasound photoacoustic theranostic system (UPTS) by combining a ultrasound system (V1, Verasonics) with a pulsed laser system. The results from the ex vivo experiments in rabbit tissues demonstrated that UPTS, by working with appropriate laser wavelengths, can selectively remove tissues such as knee tendon and liver via the cavitation synergistically created by the ultrasound bursts and the laser pulses. Such a theranostic system can deliver effective PUT treatment to biological samples along with real-time monitoring by the integrated ultrasound and photoacoustic imaging.
Photo-mediated ultrasound therapy (PUT) holds potential as a novel antivascular method. In this work, we applied PUT to precisely remove corneal neovascularization in a rabbit eye model. A stable corneal suture-induced corneal neovascularization model was established in rabbits. These rabbits were later treated by PUT or used as controls. The treatment outcomes were evaluated through red-free photography and fluorescein angiography along with histology and immunohistochemistry. The experimental results demonstrated that PUT shows promise in improving the management of eye diseases by delivering selective treatment to pathologic vessels with minimized side effects.
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