We demonstrate the label-free intra-tissue activity imaging of human induced pluripotent stem cell (hiPSC)-derived alveolar organoid including normal and fibrosis model with three-dimensional Dynamic Optical Coherence Tomography (D-OCT). The volumetric D-OCT data were obtained by a 840-nm spectral domain OCT. And the D-OCT images were obtained by “logarithmic-intensity-variance (LIV)” method, which is sensitive to the magnitude of tissue dynamics. Cystic structures with high-LIV border and mesh-like structures with low LIV were observed in all the organoids. They correspond to alveoli and fibroblast. The LIV images also visualized the abnormal repairing process of the alveolar epithelium.
KEYWORDS: Optical coherence tomography, In vitro testing, 3D modeling, Visualization, Tissues, Stereoscopy, Medical research, Lung, Drug development, Cancer
We demonstrate high-resolution (3.8-µm axial and 4.8-µm lateral) three-dimensional dynamic (D-) OCT imaging by 840-nm spectral domain OCT, and compare it with a low-resolution 1.3-µm D-OCT. The D-OCT images are obtained by logarithmic-intensity-variance method, which is sensitive to the magnitude of signal fluctuation.
Human-induced-pluripotent-stem-cell derived alveolar (lung) organoids and human breast cancer (MCF-7) spheroids were examined. The high-resolution D-OCT revealed the tessellation of high and low dynamics at the matured alveolar epithelium. It is also found that such matured alveolar epithelium exhibits ragged inner surface. For the spheroids, high-scattering spots with low dynamics were observed only in the high-resolution image.
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