The aim of this study was to assess the effect in the enamel demineralization of low-intensity infrared laser (λ=810 nm, 100 mW/cm2, 90 sec, 4.47 J/cm2, 9 J) with or without photodynamic cream fluorinated or not fluorinated, using Optical Coherence Tomography (OCT). Background data: Lasers can be used as tools for the prevention of tooth enamel demineralization.
All enamel specimens (n= 105) were analyzed using OCT at baseline, and randomly assigned into seven groups (n=15): C (+), laser application; C(-), no treatment; (F), acid fluoride gel; cream (IV); cream and neutral fluoride (IVF); cream and laser (IVL); and cream with neutral fluoride+ laser (IVFL). The specimens were submitted to all kind of treatments before demineralizing pH cycling challenge and were reanalyzed.
ANOVA and Tukey's multiple comparative analysis (p <0.01) demonstrated a greater delta attenuation between baseline and post challenge for C + (0.034 ± 0.011) compared to IVF (0.016 ± 0.007) F (0.018 ± 0.010) IVFL (0.019 ± 0.008), and IVL (0.014 ± 0.010). The cream laser group (IVL) also showed lower delta (0.014 ± 0.010) compared to C - (0.025 ± 0.008).
The OCT technique demonstrated that cream associated with laser showed the lowest quantitative enamel mineral looses after cariogenic challenge.
The in situ study evaluated antihistamine (DA) and bronchodilator(DB) drugs actions on dental enamel using FT Raman spectroscopy.
Analysis of pH drugs were permorfed, DA 1.48 and DB 2.90. Enamel (n=24) were analysed by FT - Raman and randomly distributed in control group (CG) and experimental groups (GEA and GEB), specimens fixed in palatine appliances. In CG, dripped 20% sucrose (8Xday/3 min)/7 days. In GEA, 20% sucrose (8Xday/3 min) + drug (4Xday/3 min). In GEB, 20% sucrose (8Xday/3 min) B + drug (4Xday/3 min). FT- Raman analysis was performed again. Data analyzed by Student t test and ANOVA
Differences in peak intensity of carbonate (CO3) /phosphate (PO4) (p<0.0286) post challenge in GEB related to baseline. The GEB integrated area of inorganic post challenge (p<0.0349).Organic loss in GEA, area (p<0.0228) and intensity (p<0. 0471) between moments and in GEB, area (p<0.0243) and intensity (p<0.00276). Comparing organic area and intensity difference observed post challenge GC (0,31±0,13) and GEB(0,54±0,13). Area difference between GEA(0,30±0,16) X GEB(0,54±0,13) and intensity difference GC(0,09172±0,04) and GEB(0,16±0,04) and GEA(0,091±0,05) X GEB(0,16±0,04). Significant integrated area CO3/PO4 loss for GC(0,12±0,01)X GEB(0,16±0,02) and GEB X GEA(0,13±0,02). For intensity differences were observed between GC(0,08±0,01) and GEB(0,10±0,01), and the last one and GEA(0,08±0,01)
After administration of drugs, reduction in the amount of carbonate, organic and inorganic components were observed, denoting possible demineralization.
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