Newts have a remarkable ability to regrow organs, providing key insights into the mechanisms of tissue regeneration. Lens regeneration in newts only initiates from the dorsal iris, and the underlying mechanism remains unknown. Understanding this process is critical for triggering non-competent adult tissues to support somatic cellular plasticity. Studies have focused on the difference in morphological and genetic variations between dorsal and ventral iris during the lens regeneration process using endpoint ex vivo tissue imaging and analysis, however, lens regeneration is a dynamic process. Therefore, this study aims to characterize the lens regeneration process in vivo with OCT and OCTA to non-invasively visualize the morphological variation of the anterior chamber and vasculature injury responses in the iris. Our results demonstrate that OCT is an ideal platform for in vivo monitoring lens regeneration in newts and provides a dynamic insight into this process.
OCTSharp is a versatile open-source OCT software that aims to support real-time OCT imaging development in research labs. For high-speed imaging, OCT demands high-performance real-time data processing and visualization. However, hardware control and sophisticated memory management prevent agile prototyping. The proposed OCTSharp allows users to tailor OCT for specific purposes in a short development cycle. This software is based on managed C# environments with accessible hardware control, minimal memory management, and GPU support. We demonstrate that OCT software based on C# is highly efficient and can satisfy most research-level OCT applications.
Newts have the exceptional capability of regenerating the lens through their lifetime. The transparency of the anterior chamber makes OCT an idea imaging technology to track the entire process of the lens regeneration in vivo without interruptions. We demonstrated, for the first time, that OCT can capture not only essential morphological changes similar to the changes observed in histology but some fine structures, like zonular fibers, which are not visible in histology. Our initial results warrant the future research of tailoring OCT for dynamically imaging the lens regeneration in newts.
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