Applying sparse spectral sampling SRS microscopy to evaluate topical pharmacokinetics

Isaac J. Pence; Benjamin A. Kuzma; Conor L. Evans

doi:10.1117/12.2608810

7 March 2022 Applying sparse spectral sampling SRS microscopy to evaluate topical pharmacokinetics

Isaac J. Pence, Benjamin A. Kuzma, Conor L. Evans

Proceedings Volume PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI; PC119380E (2022) https://doi.org/10.1117/12.2608810
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) is a rapid, nondestructive technique for imaging biomedical specimens with label-free chemical specificity. Here, we demonstrate a sparse spectral sampling SRS imaging approach where a rapidly-tunable dual-output all-fiber optical parametric oscillator is automatically tuned into specific vibrational modes across >1400cm-1. We demonstrate this system via simultaneously monitoring an active pharmaceutical ingredient and excipient within a single formulation, tracking penetration and permeation through distinct layers of ex vivo mouse skin over time with Raman fingerprint and high wavenumber spectral contrast. This novel method is ideally suited to monitor spatiotemporal dynamic changes during topical drug delivery.

Conference Presentation

Citation Download Citation

Isaac J. Pence, Benjamin A. Kuzma, and Conor L. Evans "Applying sparse spectral sampling SRS microscopy to evaluate topical pharmacokinetics", Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC119380E (7 March 2022); https://doi.org/10.1117/12.2608810

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