Towards high-content cell and tissue imaging with stimulated Raman scattering microscopy

Maximilian Brinkmann; Sven Dobner; Tim Hellwig; Niklas Lüpken

doi:10.1117/12.2621679

30 May 2022 Towards high-content cell and tissue imaging with stimulated Raman scattering microscopy

Maximilian Brinkmann, Sven Dobner, Tim Hellwig, Niklas Lüpken

Proceedings Volume PC12144, Biomedical Spectroscopy, Microscopy, and Imaging II; PC121440H (2022) https://doi.org/10.1117/12.2621679
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

We present our recent developments in utilizing a compact and portable light source for high-speed multicolor stimulated Raman scattering imaging (SRS) in biomedical and medical environments. The source combines a rapid and wide tunability for accessing Raman bands between 700 and 3300 cm-1 with high stability in terms of power (deviation < 0.3 %) and wavelength (deviation < 0.5 pm) over more than 100 h. We highlight applications in metabolic cell imaging and the identification of pharmaceuticals in complex environments such as skin by harvesting contrast from several Raman bands.

Conference Presentation

Citation Download Citation

Maximilian Brinkmann, Sven Dobner, Tim Hellwig, and Niklas Lüpken "Towards high-content cell and tissue imaging with stimulated Raman scattering microscopy", Proc. SPIE PC12144, Biomedical Spectroscopy, Microscopy, and Imaging II, PC121440H (30 May 2022); https://doi.org/10.1117/12.2621679

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available