Neuroscience imaging enabled by new highly tunable and high peak power femtosecond lasers

T. Hakulinen; J. Klein

doi:10.1117/12.2250407

21 February 2017 Neuroscience imaging enabled by new highly tunable and high peak power femtosecond lasers

T. Hakulinen, J. Klein

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100690D (2017) https://doi.org/10.1117/12.2250407
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Neuroscience applications benefit from recent developments in industrial femtosecond laser technology. New laser sources provide several megawatts of peak power at wavelength of 1040 nm, which enables simultaneous optogenetics photoactivation of tens or even hundreds of neurons using red shifted opsins. Another recent imaging trend is to move towards longer wavelengths, which would enable access to deeper layers of tissue due to lower scattering and lower absorption in the tissue. Femtosecond lasers pumping a non-collinear optical parametric amplifier (NOPA) enable the access to longer wavelengths with high peak powers. High peak powers of >10 MW at 1300 nm and 1700 nm allow effective 3-photon excitation of green and red shifted calcium indicators respectively and access to deeper, sub-cortex layers of the brain. Early results include in vivo detection of spontaneous activity in hippocampus within an intact mouse brain, where neurons express GCaMP6 activated in a 3-photon process at 1320 nm.

Conference Presentation

Citation Download Citation

T. Hakulinen and J. Klein "Neuroscience imaging enabled by new highly tunable and high peak power femtosecond lasers", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690D (21 February 2017); https://doi.org/10.1117/12.2250407

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available