Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes

José A. Rodrigo; Juan M. Soto; Tatiana Alieva

doi:10.1117/12.2525840

21 June 2019 Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes

José A. Rodrigo, Juan M. Soto, Tatiana Alieva

Proceedings Volume 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV; 1106016 (2019) https://doi.org/10.1117/12.2525840
Event: SPIE Optical Metrology, 2019, Munich, Germany

Abstract

Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limited-angle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a nonhomogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.

Citation Download Citation

José A. Rodrigo, Juan M. Soto, and Tatiana Alieva "Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes", Proc. SPIE 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, 1106016 (21 June 2019); https://doi.org/10.1117/12.2525840

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