9 March 2020Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Femtosecond laser-induced plasma generation is used surgically and may also cause photodamage in nonlinear microscopy. Photodamage in multiphoton microscopy already starts at irradiances 1.5 times above the value used for autofluorescence imaging but the cavitation bubble threshold is 20 x higher. We explore the realm of low-density plasma effects between multi-pulse nonlinear imaging and single-pulse surgical regime. We characterize the transition from unchanged tissue (emitting autofluorescence) to slightly changed tissue (hyperfluorescence), drastically changed tissue (plasma luminescence) and disintegrated biomolecules (gas bubble formation). By plotting the threshold values in (irradiance, radiant exposure) space, we identified a “safe” region for nonlinear microscopy.
Alfred Vogel,Xiao-Xuan Liang,Sebastian Freidank, andNorbert Linz
"Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440I (9 March 2020); https://doi.org/10.1117/12.2549643
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Alfred Vogel, Xiao-Xuan Liang, Sebastian Freidank, Norbert Linz, "Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)," Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440I (9 March 2020); https://doi.org/10.1117/12.2549643