Digital holographic microscopy with downstream biochemical analysis for in vitro cytotoxicity assessment of polymeric nanoparticles

Anne Marzi; Kai Moritz Eder; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.3003640

12 March 2024 Digital holographic microscopy with downstream biochemical analysis for in vitro cytotoxicity assessment of polymeric nanoparticles

Anne Marzi, Kai Moritz Eder, Björn Kemper, Jürgen Schnekenburger

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Proceedings Volume 12852, Quantitative Phase Imaging X; 128520J (2024) https://doi.org/10.1117/12.3003640
Event: SPIE BiOS, 2024, San Francisco, California, United States

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Abstract

Digital holographic microscopy (DHM) has been demonstrated as a label-free minimally-invasive quantitative phase imaging (QPI) tool to assess the cytotoxic potential of nanoparticles in vitro. Due to low laser light intensities DHM minimizes interactions with the sample. The analyzed cell cultures thus remain in a native state, making them available for further downstream analysis. In a study on mouse macrophages (RAW 264.7) we explored if cell populations can be analyzed with common biochemical assays after the DHM-based QPI observation. Therefore, the mean effective concentration (EC₅₀) of poly(alkyl cyanoacrylate) nanocarriers was firstly determined based on the DHM accessible dry mass increment after 24 h. The results were then compared to corresponding data from two subsequently performed colorimetric cell viability and cytotoxicity assays. EC₅₀ values from all assays are found in good agreement and demonstrate the potential of QPI to achieve an increased data quality in in vitro cytotoxicity experiments.

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Anne Marzi, Kai Moritz Eder, Björn Kemper, and Jürgen Schnekenburger "Digital holographic microscopy with downstream biochemical analysis for in vitro cytotoxicity assessment of polymeric nanoparticles", Proc. SPIE 12852, Quantitative Phase Imaging X, 128520J (12 March 2024); https://doi.org/10.1117/12.3003640

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