Optical tomography of human skin with subcellular spatial and picosecond time resolution using intense near infrared femtosecond laser pulses

Karsten Koenig; Uwe Wollina; Iris Riemann; Christiane Peukert; Karl-Juergen Halbhuber; Helga Konrad; Peter Fischer; Veronika Fuenfstueck; Tobias W. Fischer; Peter Elsner

doi:10.1117/12.470692

17 June 2002 Optical tomography of human skin with subcellular spatial and picosecond time resolution using intense near infrared femtosecond laser pulses

Karsten Koenig, Uwe Wollina, Iris Riemann, Christiane Peukert, Karl-Juergen Halbhuber, Helga Konrad, Peter Fischer, Veronika Fuenfstueck, Tobias W. Fischer, Peter Elsner

Author Affiliations +

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470692
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We describe the novel high resolution imaging tool DermaInspect 100 for non-invasive diagnosis of dermatological disorders based on multiphoton autofluorescence imaging (MAI)and second harmonic generation. Femtosecond laser pulses in the spectral range of 750 nm to 850 nm have been used to image in vitro and in vivo human skin with subcellular spatial and picosecond temporal resolution. The non-linear induced autofluorescence originates mainly from naturally endogenous fluorophores/protein structures like NAD(P)H, flavins, keratin, collagen, elastin, porphyrins and melanin. Second harmonic generation was observed in the stratum corneum and in the dermis. The system with a wavelength-tunable compact 80 MHz Ti:sapphire laser, a scan module with galvo scan mirrors, piezoelectric objective positioner, fast photon detector and time-resolved single photon counting unit was used to perform optical sectioning and 3D autofluorescence lifetime imaging (t-mapping). In addition, a modified femtosecond laser scanning microscope was involved in autofluorescence measurements. Tissues of patients with psoriasis, nevi, dermatitis, basalioma and melanoma have been investigated. Individual cells and skin structures could be clearly visualized. Intracellular components and connective tissue structures could be further characterized by tuning the excitation wavelength in the range of 750 nm to 850 nm and by calculation of mean fluorescence lifetimes per pixel and of particular regions of interest. The novel non-invasive imaging system provides 4D (x,y,z,t) optical biopsies with subcellular resolution and offers the possibility to introduce a further optical diagnostic method in dermatology.

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Karsten Koenig, Uwe Wollina, Iris Riemann, Christiane Peukert, Karl-Juergen Halbhuber, Helga Konrad, Peter Fischer, Veronika Fuenfstueck, Tobias W. Fischer, and Peter Elsner "Optical tomography of human skin with subcellular spatial and picosecond time resolution using intense near infrared femtosecond laser pulses", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470692

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