In vitro effects of 635 nm low intensity diode laser irradiation on the fat distribution of one adipose cell

Efrain Solarte; Carolina Isaza; Willian Criollo; Aldo F. Rebolledo; Jose Arroyave; Hugo Ramirez; Rodrigo Neira

doi:10.1117/12.527513

19 November 2003 In vitro effects of 635 nm low intensity diode laser irradiation on the fat distribution of one adipose cell

Efrain Solarte, Carolina Isaza, Willian Criollo, Aldo F. Rebolledo, Jose Arroyave, Hugo Ramirez, Rodrigo Neira

Author Affiliations +

Proceedings Volume 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life; (2003) https://doi.org/10.1117/12.527513
Event: 19th Congress of the International Commission for Optics: Optics for the Quality of Life, 2002, Florence, Italy

Abstract

In vitro effects of 635-nm diode laser irradiation on the lipidic inclusions and the cellular fat distribution were observed in situ on a selected multilocular adipose cell in culture by an effective laser power of 3.16 mW. Selected microscopic field was 12 times sequentially irradiated, using 100 seconds exposures, a free spot of 5 mm and effective energy density of 1.6 Jcm^-2 per exposure. Same field was irradiated 24 times using a beam spot of 10 mm, 0.4 Jcm^-2. Digital microphotograph sequences permit to observe and follow changes in fat distribution. Results show changes in fat vesicles. Microscopic follow-up shows an almost empty vacuole 15 hours after irradiation, the cell was empty after 42 hours, and dies after 52 hours.

Citation Download Citation

Efrain Solarte, Carolina Isaza, Willian Criollo, Aldo F. Rebolledo, Jose Arroyave, Hugo Ramirez, and Rodrigo Neira "In vitro effects of 635 nm low intensity diode laser irradiation on the fat distribution of one adipose cell", Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); https://doi.org/10.1117/12.527513

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