Limitation of detection of microarray analyzer based on CCD

Guoxiong Xu; Yan Shi; Xuxiang Ni; Zukang Lu

doi:10.1117/12.574915

18 January 2005 Limitation of detection of microarray analyzer based on CCD

Guoxiong Xu, Yan Shi, Xuxiang Ni, Zukang Lu

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.574915
Event: Photonics Asia, 2004, Beijing, China

Abstract

The most successful biochip technologies today are flat microarray and suspension microarray. Usually probes are fluorescence labeled. The fluorophores are excited by laser with a special wavelength. Because the fluorescence signal is very weak, it is hard to detect. The limitation of detection (LOD) is an important index of microarray analyzer. The dependence of LOD of flat and suspension microarray analyzer based on CCD and the fluorescent intensity on characters of excitation light optical system and fluorescence collection optical system as well as the parameters of elements system has been analyzed in detail based on the system configuration. A formula of LOD and fluorescence signal intensity depending on those parameters has been established. The study analyzed system limitation of detection (LOD). Also present a formula of minimal detectable fluorescent molecule numbers as the function of each parameter of microarray analyzer based on CCD. Estimated LOD of our suspension microarray detection system is about 7.9 fluorophores/μm² at exposure time 1s.

Citation Download Citation

Guoxiong Xu, Yan Shi, Xuxiang Ni, and Zukang Lu "Limitation of detection of microarray analyzer based on CCD", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.574915

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