Full-color three-dimensional microscopy using white-light interference on a color camera

Lingfeng Yu; Myung K. Kim

doi:10.1117/12.614257

29 August 2005 Full-color three-dimensional microscopy using white-light interference on a color camera

Lingfeng Yu, Myung K. Kim

Proceedings Volume 5875, Novel Optical Systems Design and Optimization VIII; 58750L (2005) https://doi.org/10.1117/12.614257
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

We present experimental demonstration of a novel full-color three-dimensional microscopy method. A white-light high-brightness LED illuminates a Michelson interferometer. A color CCD camera records the color image of an object in superposition with the reference beam. The short-coherence interferometric process is applied to each of the three color channels to extract tomographic contour images of the object. The set of three images are recombined as RGB channels to construct a color tomographic image of a section of the object. The reference mirror is scanned over a range of axial distances to construct a three-dimensional tomographic image of the object with full natural color representation. The technique is applied to imaging of artificial color objects as well as colorful biological objects with about 10 um axial resolution, about 100 ~ 200 um penetration depth, and 50 ~ 60 db dynamic range. In contrast to most existing three-dimensional microscopy methods, the present technique allows monitoring of tissue structures close to its natural color, which may be useful in various physiological and pathological applications.

Citation Download Citation

Lingfeng Yu and Myung K. Kim "Full-color three-dimensional microscopy using white-light interference on a color camera", Proc. SPIE 5875, Novel Optical Systems Design and Optimization VIII, 58750L (29 August 2005); https://doi.org/10.1117/12.614257

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KEYWORDS

Microscopy

3D image processing

Optical coherence tomography

Light emitting diodes

RGB color model

Tomography

Cameras

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