Quantitative analysis of urinary stone composition with micro-Raman spectroscopy

Yi-Yu Huang; Yi-Chun Chiu; Huihua Kenny Chiang; Y. H. Jet Chou; Shing-Hwa Lu; Allen W. Chiu

doi:10.1117/12.841723

2 March 2010 Quantitative analysis of urinary stone composition with micro-Raman spectroscopy

Yi-Yu Huang, Yi-Chun Chiu, Huihua Kenny Chiang, Y. H. Jet Chou, Shing-Hwa Lu, Allen W. Chiu

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75480X (2010) https://doi.org/10.1117/12.841723
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Urolithiasis is a common, disturbing disease with high recurrent rate (60% in five years). Accurate identification of urinary stone composition is important for treatment and prevention purpose. Our previous studies have demonstrated that micro-Raman spectroscopy (MRS)-based approach successfully detects the composition of tiny stone powders after minimal invasive urological surgery. But quantitative analysis of urinary stones was not established yet. In this study, human urinary stone mixed with two compositions of COM, HAP, COD, and uric acid, were analyzed quantitatively by using a 632.98 nm Raman spectrometric system. This quantitative analysis was based on the construction of calibration curves of known mixtures of synthetically prepared pure COM, HAP, COD and uric acid. First, the various concentration (mole fraction) ratio of binary mixtures including COM and HAP, COM and COD, or COM and uric acid, were produced. Second, the intensities of the characteristic bands at 1462cm ^-1(I_RCOM), 1477cm^-1(I_RCOD), 961cm^-1(IRHAP) and 1402cm^-1(I_{Ruric acid}), for COD, COM, HAP and uric acid were used respectively for intensity calculation. Various binary mixtures of known concentration ratio were recorded as the basis for the quantitative analysis. The ratios of the relative intensities of the Raman bands corresponding to binary mixtures of known composition on the inverse of the COM concentration yielded a linear dependence. Third, urinary stone fragments collected from patients after management were analyzed with the use of the calibration curve and the quantitative analysis of unknown samples was made by the interpolation analysis. We successfully developed a MRS-based quantitative analytical method for measuring two composition.

Citation Download Citation

Yi-Yu Huang, Yi-Chun Chiu, Huihua Kenny Chiang, Y. H. Jet Chou, Shing-Hwa Lu, and Allen W. Chiu "Quantitative analysis of urinary stone composition with micro-Raman spectroscopy", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480X (2 March 2010); https://doi.org/10.1117/12.841723

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Raman spectroscopy

Quantitative analysis

Binary data

Calibration

Micro raman spectroscopy

Spectroscopy

Statistical analysis

Show All Keywords

Keywords/Phrases

Search In:

Publication Years