Expression and function of channelrhodopsin 2 in mouse outer hair cells

Fangyi Chen; Tao Wu; Teresa Wilson; Hrebesh Subhash; Irina Omelchenko; Michael Bateschell; Lingyan Wang; John Brigande; Zhi-Gen Jiang; Alfred Nuttall

doi:10.1117/12.2003114

8 March 2013 Expression and function of channelrhodopsin 2 in mouse outer hair cells

Fangyi Chen, Tao Wu, Teresa Wilson, Hrebesh Subhash, Irina Omelchenko, Michael Bateschell, Lingyan Wang, John Brigande, Zhi-Gen Jiang, Alfred Nuttall

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Proceedings Volume 8586, Optogenetics: Optical Methods for Cellular Control; 85860I (2013) https://doi.org/10.1117/12.2003114
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Outer hair cell (OHC) is widely accepted as the origin of cochlear amplification, a mechanism that accounts for the extreme sensitivity of the mammalian hearing. The key process of cochlear amplification is the reverse transduction, where the OHC changes its length under electrical stimulation. In this study, we developed a method to modulate electro-mechanical transduction with an optogenetic approach based on channelrhodopsin 2 (ChR2), a direct lightactivated non-selective cation channel (NSCC). We specifically expressed ChR2 in mouse cochlea OHCs through in uterus injection of adenovirus vector with ChR2 in fusion with the fluorescent marker tdTomato. We also transfected ChR2(H134R), a point mutant of ChR2, with plasmid to an auditory cell line (HEI-OC1). With whole cell recording, we found that blue light (470 nm) elicited a current with a reversal potential around zero in both mouse OHCs and HEI-OC1 cells and generated depolarization in both cell types.

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Fangyi Chen, Tao Wu, Teresa Wilson, Hrebesh Subhash, Irina Omelchenko, Michael Bateschell, Lingyan Wang, John Brigande, Zhi-Gen Jiang, and Alfred Nuttall "Expression and function of channelrhodopsin 2 in mouse outer hair cells", Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860I (8 March 2013); https://doi.org/10.1117/12.2003114

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KEYWORDS

Optogenetics

Systems modeling

Ear

Fluorescent markers

Uterus

Axons

Light emitting diodes

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